Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous c...

Understanding the biological potential of fetal stem/progenitor cells will help define mechanisms in liver development and homeostasis. We isolated epithelial fetal human liver cells and established phenotype-specific changes in gene expression during continuous culture conditions. Fetal human liver epithelial cells displayed stem cell properties with multilineage gene expression, extensive pro...

background: in this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. materials and methods: human umbilical cord blood-derived mesenchymal stem cells were first cultured in dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incub...

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...

All important developmental milestones are accomplished during the fetal stage, and nutrient fluctuation during this stage produces lasting effects on offspring health, so called fetal programming or developmental programming. The fetal stage is critical for skeletal muscle development, as well as adipose and connective tissue development. Maternal under-nutrition at this stage affects the prol...

Introduction: Human hematopoietic stem cells (hHSCs) have been used for transplantation in hematologic failures. Because the number of hHSCs per cord blood unit is limited, the expansion of these cells is important for clinical application. It has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hHSCs. In this regard, cord blood CD34+ cells ex...

background: keratinocyte stem cell is one of the adult stem cells that inhabits the skin and contributes to skin function and renewal. adult stem cells are best defined by their capacity to self-renew, and to maintain tissue function for a long period of time. these findings indicate the importance of these cells for clinical applications including regenerative medicine, tissue engineering and ...

background: several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (mscs) in allogeneic or mitogenic interactions. cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. objective: to investigate if adipose derived mscs could inhibit jurkat lym-phoblastic leukemia t cell proliferation during coculture. me...

We envision the use of human fetal bone cells for engineered regeneration of adult skeletal tissue. A description of their cellular function is then necessary. To our knowledge, there is no description of human primary fetal bone cells treated with differentiation factors. The characterization of fetal bone cells is particularly important as the pattern of secreted proteins from osteoblasts has...