Aspiration of equine sternal bone marrow is required for the cultivation of bone marrow-derived multipotent mesenchymal stromal cells (BM-MSCs) for regenerative therapies. For bone marrow aspiration as well as for MSC cultivation, there is a need to optimize techniques and protocols to enhance MSC harvest at minimized culture times. In a comparative study bone marrow aspirates from sternebra 4 ...

The regulation of cyclic adenosine 3':5'-monophosphate (cAMP)-binding protein in N-18 neuroblastoma cells in tissue culture was studied by the covalent incorporation of 8-azido-cyclic adenosine 3':5'-[32P]monophosphate, together with the techniques of sodium dodecyl sulfate:polyacrylamide gel electrophoresis and autoradiography. Greater than 95% of the total cAMP binding activity of N-18 neurob...

PURPOSE To determine, in vitro, the susceptibility to apoptosis of lymphocytes from patients with peripheral arterial disease (PAD) in the presence of a low culture medium serum concentration, and to evaluate the correlation of the degree of apoptosis and the serum lipid levels. METHODS Lymphocytes were isolated from the venous blood of PAD patients with lower limb ischemia secondary to oblit...

Hyaluronic acid (HA) was extracted from the cell layer of cultured mouse dermal fibroblasts with 6 M guanidine HCl in the presence of 8% (w/v) Zwittergent. HA could be separated from the bulk of extracted proteins by consecutive isopycnic centrifugation and gel and ion-exchange chromatography under dissociative conditions. The final preparation was the complex of HA (viscosity average molecular...

A system for culturing human fetal liver cells in monolayers is described and the effects of various conditions of growth on the morphology and function of the cultured cells are presented. The addition of 10% calf serum or 1% human serum to the growth medium accelerated the proliferation of the liver cells, with subsequent loss of characteristic morphology and specific functional activity. In ...

Macrophages are responsible for removing the extravasated blood that forms a bruise. Aspects of this process, such as how long it takes erythrocytes to be phagocytosed and iron demonstrated using Perls stain, not established. This work sought assess possibility macrophages in cell culture address some unknowns processes resolution Human peripheral monocytes were obtained from buffy coats provid...

background: pregnancy and accelerated fetal growth always are related with major metabolic changes and body fat redistribution and adiponectin is one of principle adipocyte hormones, so studying adiponectin changes during pregnancy may reveal some hidden parts of fetal metabolism. the aim of this study was to assess adiponectin and leptin levels in umbili cal cord and maternal serum, their rela...

REEVES, JOHN T., FUHEID S. DAOIJD, AND MICHAEL GENTRY. Growth of the fetal calf and its arterial pressure, blood gases, and hematologic data. J. Appl. Physiol. 32(2): 240-244. 1972.-Fetal growth, arterial pressure, arterial oxygen tensions, and hematologic data in 35 fetal calves were compared with similar data in fetal lambs. The data of the present report suggested that the fetal calf, its or...

Insulin-like growth factors I and I1 (IGF-I and IGF11) were prepared from fetal calf serum and adult bovine serum by gel filtration, immunoaffinity chromatography, chromatofocusing, and reverse-phase high pressure liquid chromatography, and their complete amino acid sequences determined. IGF-I and -11 are found in both adult and fetal serum. The sequence of bovine IGF-I is found to be identical...