Fermentation of sugar by Saccharomyces cerevisiae, for production of ethanol in an immobilized cell reactor (ICR) was successfully carried out to improve the performance of the fermentation process. The fermentation set-up was comprised of a column packed with beads of immobilized cells. The immobilization of S. cerevisiae was simply performed by the enriched cells cultured media harvested at e...

Polyhydroxyalkanoates (PHAs) are biological polyesters produced through microbial fermentation processes. They have attracted attention as an alternative source to petro-chemically derived plastics as they are biodegradable, renewable, biocompatible and environmentally friendly. However, a notable limitation for their bulk production is the producer microbes’ low yield and productivity which le...

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is h...

The flow cytometry (FC) technique used with certain fluorescent dyes (ChemChrome V6 [CV6], DRAQ5, and PI) has proven useful to label and to detect different physiological states of yeast and malolactic bacterium starters conducting cider fermentation over time (by performing sequential inoculation of microorganisms). First, the technique was tested with pure cultures of both types of microorgan...

Several aspects concerning the bioconversion of xylose-containing hydrolysates (obtained from Eucalyptus wood) into xylitol were assessed. Debaryomyces hansenii yeast strains were adapted to fermentation media (obtained either by prehydrolysis or autohydrolysis-posthydrolysis of wood) supplemented with low-cost nutrients. Media containing up to 80 g/L xylose were efficiently fermented when the ...

Yeast is a unicellular fungi that does not reproduce via asexual spore production (Phaff, 1966). The most commonly fed yeast in dairy diets is Saccharomyces cerevisiae (SC); a facultative anaerobic yeast often referred to as brewers or bakers yeast. The most common yeast products fed to ruminants are live cells or yeast culture mixtures. A yeast culture is a yeast-fermented product that contain...

Pertussis toxin (Ptx) expression and secretion in Bordetella pertussis are regulated by a two-component signal transduction system encoded by the bvg regulatory locus. However, it is not known whether the metabolic pathways and growth state of the bacterium influence synthesis and secretion of Ptx and other virulence factors. We have observed a reduction in the concentration of Ptx per optical ...

Among the various fungal strains screened for citric acid production, Aspergillus niger is known to produce considerable amounts of citric acid and other organic acids when cultivated in carbohydrate-rich medium of fermentation. A number of indigenous raw materials such as cane molasses and pumpkin have been carried out in present study for citric acid production by using gamma ray induced muta...

Microorganisms have provided a bounty of bioactive secondary metabolites with very exciting biological activities such as antibacterial, antifungal antiviral, and anticancer, etc. The present study aims at the optimization of culture conditions for improved antimicrobial production of Paenibacillus elgii obtained from Wayanad forest of Western Ghats region of Kerala, India. A bacterial strain i...