FEN-1 proteins are a family of nucleases essential for lagging strand DNA synthesis. A gene with sequence similarity to FEN-1 protein-encoding genes, rad2 +, has been identified in Schizosaccharomyces pombe . We report the overexpression, purification, and character-ization of the putative S.pombe FEN-1 homolog, Rad2p. A GST-Rad2p fusion protein was over-expressed in Saccharomyces cerevisiae an...

It is not well understood how the ecological status and microbial community composition of spruce swamp forests (SSF) relate to those found in bogs and fens. To clarify this, we investigated biogeochemical parameters and microbial community composition in a bog, a fen and two SSF using high throughput barcoded sequencing of the small ribosomal subunit (SSU) variable region V4. The results demon...

This paper presents some of Santorio's marginalia to his Commentaria in primam fen primi libri Canonis Avicennae (Venice, 1625), which I identified in the Sloane Collection of the British Library in 2016, as well as the evidence for their authorship. The name of the Venetian physician Santorio Santori (1561-1636) is linked with the introduction of quantification in medicine and with the inventi...

We prove existence results for the solutions of the periodic boundary value problem concerning the n-th order functional differential equation with impulses effects ⎩ ⎨ ⎧ Δ ∈ α α − , p , 1, = k)), t (x ,), t (x (I =) t (x ], T [0, t. e. a))), t ((x ,)) t ((x), t (x , t (f =) t (x k 1) n (k k , i k) i (m 1) n (L L L and the periodic boundary conditions 1. , 0, =), (= (0)) () (− n i T x x i i L O...

OBJECTIVE: VIP and PACAP, two structurally related peptides, stimulate c AMP, steroidogenesis and progesterone (PROG) release from cultured rat granulosa cells. VIP and PACAP38 are known to mimic the effects of β-adrenergic receptor stimulation in the rat pinealocytes causing an increased melatonin synthesis. These effects were markedly potentiated by α1 adrenergic receptor stimulation. METHODS...

MOTIVATION Of the approximately 200 proteins that have been identified for the vaccinia virus (VACV) genome, many are currently listed as having an unknown function, and seven of these are also found in all other poxvirus genomes that have been sequenced. The G5R protein of VACV is included in this list, and to date, very little is known about this essential and highly conserved protein. Conven...

Wetlands represent sources or sinks of the greenhouse gas nitrous oxide (N2O). The acidic fen Schlöppnerbrunnen emits denitrification derived N2O and is also capable of N2O consumption. Global warming is predicted to cause more extreme weather events in future years, including prolonged drought periods as well as heavy rainfall events, which may result in flooding. Thus, the effects of prolonge...

background: perforin is known to be important in cytolytic activity mediated by natural killer (nk) cells.   objective: to study the relationship between the efficiency of nk and lymphokine-activated killer (lak) cells activity, and the expression of perforin and hla class i molecules.   methods: lak cells were generated by in vitro culturing of human peripheral blood lymphocytes (pbls) in the ...

Measuring Responsiveness in the Therapeutic Relationship: A Patient Perspective Harry T. Reis , Margaret S. Clark , Denis J. Pereira Gray , Fen-Fang Tsai , Judith B. Brown , Moira Stewart & Lynn G. Underwood To cite this article: Harry T. Reis , Margaret S. Clark , Denis J. Pereira Gray , Fen-Fang Tsai , Judith B. Brown , Moira Stewart & Lynn G. Underwood (2008) Measuring Responsiveness in the ...