PURPOSE To evaluate training induced metabolic changes noninvasively with (31)P magnetic resonance spectroscopy ((31)P-MRS) for measuring muscle fibre type adaptation. METHODS Eleven volunteers underwent a 24-week training, consisting of speed-strength, endurance, and detraining (each 8 weeks). Prior to and following each training period, needle biopsies and (31)P-MRS of the resting gastrocne...

Today COVID-19 pandemic caused by the SARS-CoV-2 virus is most challenging health issue due to fast transmission rate and its impact on different aspects of human life. Although RT-PCR primary method detect SARS-CoV-2, other reliable methods are being developed this pathogen. Biosensors can provide fast, reliable, point-of-care diagnostic. Among them, electrochemical biosensors attract consider...

Phosphorus ((31) P) MRS, combined with saturation transfer (ST), provides non-invasive insight into muscle energy metabolism. However, even at 7 T, the standard ST method with T1 (app) measured by inversion recovery takes about 10 min, making it impractical for dynamic examinations. An alternative method, i.e. four-angle saturation transfer (FAST), can shorten the examination time. The aim of t...

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tube...

An evaluation is made of the complementarity between two non-invasive techniques, cuticle microhistological analysis (CMA) and PCR-capillary electrophoresis (PCR-CE) DNA-based analysis, for determination herbivore diet composition from faecal samples. Cuticle based on different microanatomical characteristics epidermal fragments remaining in faeces. The PCR-CE technique combines PCR amplificati...

Biopsy specimens were obtained from 102 leprosy patients before chemotherapy and examined by polymerase chain reaction (PCR) using the primers amplifying the 372-bp DNA of a repetitive sequence of Mycobacterium leprae. The PCR results were then compared with bacterial indices (BI) of slit-skin smears and biopsy specimens. The intensities of DNA bands were in general correlated with the numbers ...

INTRODUCTION Tuberculosis (TB) causes significant morbidity and mortality worldwide as one of the leading infectious diseases. In India, more than 1.8 million new cases occur every year. Rapid and accurate diagnosis of TB would improve patient care and limit its transmission. This study aimed to evaluate a dual target polymerase chain reaction (PCR) diagnostic assay to detect Mycobacterium tube...