Manchurian ash (Fraxinus mandshurica Rupr.) is a valuable hardwood species in Northeast China. In cultures of F. mandshurica, somatic embryos were produced mainly on browned explants. Therefore, we studied the mechanism of explant browning and its relationship with somatic embryogenesis (SE). We used explants derived from F. mandshurica immature zygotic embryo cotyledons as materials. Proteins ...

In order to elucidate the role of parathyroid hormone-related peptide (PTHrP) in tooth development, we treated tooth germ explants of mouse molars with antisense phosphorothioate-oligodeoxynucleotide (ODN) against PTHrP. Antisense ODN-treatment of the explants resulted in the invasion of the tooth germs by bone. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells around the ...

Background: Enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli epithelial cell adhesion is characterised by intimate attachment, and attaching and effacing (A/E) lesion formation. This event is mediated in part by intimin binding to another bacterial protein, Tir (translocated intimin receptor), which is exported by the bacteria and integrated into the host cell plasma membr...

We have succeeded in the isolation, culture and morphological characterization of Rana ridibunda stomach enteric plexuses. We have furthermore obtained intra and extracellular bioelectric recordings from the explants in culture. The culture medium used (Eagle MEM), the collagenase digestion and the general culture conditions followed are similar to those applied to mammal enteric plexus explant...

Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requiremen...

Regular polygonal networks have been found in explants and dissociated cells of early chick embryos. These networks are readily observable in live cells with phase-contrast optics thus allowing time-lapse cinemicroscopy. They consisted of a regular pattern of nodes and radiating struts found predominantly in the lamelliplasm of the free edges of the cells bordering explants. At the outer edge, ...

A rapid Agrobacterium tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. The explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the [beta]-glucuronidase gene with an intron, and a selectable marker, the n...

Interstitial cells of Cajal (ICC) play a critical role in the control of gastrointestinal motility as pacemaker cells and as regulators of enteric innervation. ICC are one of the first cell types that are injured during an inflammatory process and maintenance of ICC health or promotion of growth and development maybe crucial in recovery after injury. The aim of this study was to evaluate the ro...

of embryogenic frequency 88.9 and 72.23 were recorded for stem explants. However, the greatest embryogenic calli fresh weights 14.68 and 9.65 (mg/jar) were derived from leaf explants. Moreover, growth of somatic embryos was investigated during 7weeks. The highest regeneration percentage was achieved for stem explants compared other explants and recorded 88.88 and 66.66 for directly or indirectl...

A new method for culturing spinal cord slices or explants is presented which entails the use of a commercially available purified collagen, Vitrogen. Vitrogen provides a stable three-dimensional matrix for culturing spinal cord explants which is superior to the conventional method of applying explants to moist dishes coated with rat tail collagen. The use of Vitrogen facilitated the culturing o...