The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

abstract to identify the species, understanding the characteristics of the species, explore the possibility of speciation, mutations in the study population, and many other purposes. in this method for studying colchesin bath, karyotype use for immature worms. after reviewing the slide karyotype of polychaeta (nereis sp.) and observation and analysis of  50  plaque metaphase plate chromosome nu...

Inherently dynamic, chromosomes adopt many different conformations in response to DNA metabolism. Models of chromosome organization in the yeast nucleus obtained from genome-wide chromosome conformation data or biophysical simulations provide important insights into the average behavior but fail to reveal features from dynamic or transient events that are only visible in a fraction of cells at ...

aegilops triuncialis l. (2n=4x=28; ctctutut) is one of the tetraploid aegilops species harboring valuable genes for resistance to many biotic and abiotic stresses. in the present study the bread wheat cultivars mv17, navid, omid, pishgam, zarin were crossed with an accession of ae. triuncialis and the resulted f1 and f2 (obtained by the selfing of f1 plants) hybrids studied using the convention...

abstract background: to produce a reliable probe suitable for aneuploidy detection of chromosome 13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization (fish), we used a contig of three overlapping cosmids mapped to 13q12.3. methods: the cosmid dna carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin-11-dutp. the...

background: the sperm count and function may be affected by karyotype abnormalities or microdeletion in y chromosome. these genetic abnormalities can probably transmit to the children. objective: in this study, we tried to determine the frequency of karyotype abnormalities and y chromosome microdeletions in severe oligospermic or azoospermic men who fathered sons by icsi. materials and methods:...

least squares method (lsm) is an accurate and rapid method for solving some analytical andnumerical problems. this method can be used to estimate the average reservoir pressure in well testanalysis. in fact, it may be employed to estimate parameters such as permeability (k) and porevolume (vp). regarding this point, buildup, drawdown, late transient test data, modified muskatmethod, interferenc...

Drosophila mauritiana is an Indian Ocean island endemic species that diverged from its two sister species, Drosophila simulans and Drosophila sechellia, approximately 240,000 years ago. Multiple forms of incomplete reproductive isolation have evolved among these species, including sexual, gametic, ecological, and intrinsic postzygotic barriers, with crosses among all three species conforming to...