/3-Ketoacyl-acyl carrier protein (ACP) synthetase catalyzes the condensation reaction of fatty acid synthesis in Escherichia coli. The homogeneous enzyme reacts with hexanoylCoA to form hexanoyl-enzyme which was isolated and characterized. Hexanoyl-enzyme contains 2 mol of hexanoate/mol of enzyme (molecular weight 66,000); it is labile at alkaline pH, and it reacts with neutral hydroxylamine to...

Abstract Gluten-free beer (GFB) was produced from gluten-containing barley malt wort by adding an enzyme-enriched extract to the wort, intended for patients with celiac disease. Nevertheless, aroma profile of such is unknown. The results indicated that treatment reduced 3-methylbutyl acetate, 2-methylbutyl ethyl hexanoate, 2-methylpropyl and butyrate when compared reference. Moreover, foam, fre...

Hanseniaspora guilliermondii and Hanseniaspora uvarum were tested in grape must fermentations as pure and mixed starter cultures with Saccharomyces cerevisiae. In pure cultures, the specific growth rates found were 0.29 h(-1) for H. uvarum, 0.23 h(-1) for H. guilliermondii and 0.18 h(-1) for S. cerevisiae. No significant differences were observed between these values and those obtained in mixed...

Nineteen host plant volatiles (HPVs) were screened for attractivity to adult codling moth Cydia pomonella (L.) as a fourth component of core blends (3K) including (E,Z)-2,4-ethyl decadienoate, (E)-4,8-dimethyl-1,3,7-nonatriene and acetic acid. Each new quaternary combination was compared with previously reported attractive bisexual lure (4K), consisting the 3K blend plus 6-ethenyl-2,2,6-trimeth...

The interest in new beer products, which has been growing for several years, forces technologists and brewers to look innovative raw materials, such as hops, sources of carbohydrates or yeast. aim the presented study was evaluate effect selected Saccharomyces (Saccharomyces paradoxus (CBS 7302), S. kudriavzevii 3774), cerevisiae (Safbrew T-58)) non-Saccharomyces yeast (W. anomalus 5759), Ha. uv...

Poly(vinylidene fluoride), PVDF, membranes have attracted considerable attention as polymer electrolytes for fuel cells. This study explores the effect of solvent on spherulite size and crystallinity polymeric membranes. Based Hansen solubility parameters theory, mixture DMC DMSO was selected among a dozen solvents preparation PVDF by thermally induced phase separation. The addition two protic ...

The alkaline cold-active lipase from Pseudomonas fluorescens AMS8 undergoes major structural changes when reacted with hydrophobic organic solvents. In toluene, the AMS8 lipase catalytic region is exposed by the moving hydrophobic lid 2 (Glu-148 to Gly-167). Solvent-accessible surface area analysis revealed that Leu-208, which is located next to the nucleophilic Ser-207 has a focal function in ...

The proton-coupled folate transporter (PCFT) was recently identified as the major uptake route for dietary folates in humans. The three-dimensional structure of PCFT and its detailed interplay with function remain to be determined. We screened the water-accessible extracellular surface of HsPCFT using the substituted-cysteine accessibility method, to investigate the boundaries between the water...

1. The oxidation of butyrate, hexanoate and octanoate by rat-liver mitochondria suspended in a tris-potassium chloride medium in the presence of malate and serum albumin has been investigated. 2. The oxidation of butyrate to acetoacetate was markedly decreased by the addition of a system competitive for ATP (hexokinase-glucose). 3. Serum albumin or tricarboxylic acid-cycle intermediates prevent...