A method for achieving strand specific nicking of DNA has been developed. Phosphorothioate groups were incorporated enzymatically into the (-)strand of M13 RF IV DNA. When such DNA is reacted with restriction endonucleases in the presence of ethidium bromide nicked DNA (RF II) is produced. All of the restriction enzymes tested linearised phosphorothioate-containing DNA in the absence of this dy...

Adult Mice (BWt. 30 gms and 40gms) were treated at a 5mg\kgBWt. and 10 mg/kg BWt. of Ethidium Bromide for 10 days in drinking water. Control animals were given equal dose of deionised water. Quantitative and qualitative changes were studied in Spleen. The following features were evaluated: the overall cellularity of the spleen was noticed. The changes were characterised by, injury to spleen tis...

A meiotic segregant (oliPR1) was isolated with a phenotype of multiple cross resistance and collateral sensitivity. Strain oliPR1 has increased sensitivity to ethidium bromide, dequalinium chloride, acriflavin, paromomycin and neomycin, and increased resistance to oligomycin, rutamycin, venturicidin, triethyltin bromide, antimycin, carbonylcynamide-m-chlorophenylhydrazone, tetra-N-butylammonium...

Molecular Probes’ new generation of fluorescent nucleic acid gel stains — the SYBR Gold, SYBR Green I and SYBR Green II dyes — are by far the best high-sensitivity reagents for staining DNA (Figure 8.60) and RNA (Figure 8.61) in electrophoretic gels.1 These gel stains provide greater sensitivity with lower background fluorescence than the conventional gel stain, ethidium bromide. In addition, A...

HeLa SO cells were synchronized by selective mitotic detachment and chromatin was isolated from synchronized populations at various times during the cell cycle. The isolated chromatins were studied by circular dichroism and for their ability to bind the intercalating dye, ethidium bromide. Chromatin from cells in the middle of the S phase showed an increase in maximum ellipticity in the 250to 3...

Antibiotic resistance has become a global health problem for humankind. Improper use of antibiotics resulted in the increasing evolved bacterial to them. There are different types mechanisms including efflux pumps. To overcome pump activity on drugs, combinatorial therapy existing antimicrobials with natural products is promising insight prevent multidrug resistance. In this study, inhibitory a...

The abnormal expansion of unstable simple sequence DNA repeats can cause human disease through a variety of mechanisms, including gene loss-of-function, toxic gain-of-function of the encoded protein and toxicity of the repeat-containing RNA transcript. Disease-associated unstable DNA repeats display unusual biophysical properties, including the ability to adopt non-B-DNA structures. CAG•CTG tri...