UNLABELLED PREMISE OF THE STUDY A set of expressed sequence tag (EST) microsatellite markers were developed and characterized using next-generation sequencing technology for the Chinese genus Ostryopsis (Betulaceae). • METHODS AND RESULTS A total of 38 high-quality simple sequence repeat (SSR) primers were identified, of which 15 could be successfully amplified. Subsequently, we selected 8...

Mining for microsatellites (also called simple sequence repeats [SSRs]) in public sequence databases of a common Indo-Pacific coral Acropora millepora identified 191 SSRs from 10 258 expressed sequence tag (EST) and 618 SSRs from 14 625 whole-genome shotgun (WGS) sequences. In contrast to other animals, trinucleotide repeats, rather than dinucleotide repeats, are dominant in the WGS-SSRs, and A...

S. N. Qureshi, Department of Plant and Soil Science, P.O. Box 9555, Mississippi State University, Mississippi State, MS 39762; S. Saha and J. N. Jenkins, USDA-ARS, Crop Science Research Laboratory, P.O. Box 5367, Mississippi State, MS 39762; Ramesh V. Kantety, Department of Crop & Soil Sciences, 726 Bradfield Hall, Cornell University, Ithaca, NY 14853 * Corresponding author: [email protected]...

With a significant economic value, pepper (Capsicum annuum L., Solanaceae) represents major vegetable crop worldwide. Simple sequence repeats (SSR) markers and expressed tags (EST-SSR) are powerful tools for genetic studies the breeding of pepper. In this study, variability among local species was assessed by using 8 EST/SSR markers. The varieties were collected locally, based on old sorts, hyb...

New source of molecular markers accelerate the efforts in improving cotton fiber traits and aid in developing high-density integrated genetic maps. We developed new markers based on candidate genes and G. arboreum EST sequences that were used for polymorphism detection followed by genetic and physical mapping. Nineteen gene-based markers were surveyed for polymorphism detection in 26 Gossypium ...

Mung bean (Vigna radiate (L.) Wilczek) is an important traditional food legume crop, with high economic and nutritional value. It is widely grown in China and other Asian countries. Despite its importance, genomic information is currently unavailable for this crop plant species or some of its close relatives in the Vigna genus. In this study, more than 103 million high quality cDNA sequence rea...

Turmeric (Curcuma longa L., family Zingiberaceae) is one of the most economically important plants for its use in food, medicine, and cosmetic industries. Cultivar identification is a major constraint in turmeric, owing to high degree of morphological similarity that in turn, affects its commercialization. The present study addresses this constraint, using EST-SSR marker based, molecular identi...