introduction : toxoplasmosis is a parasitic infection caused by the protozoan toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. toxoplasma tissue cyst matrix protein (mag1) induces early humoral and cell-mediated immune responses. previous studies suggested recombinant mag...

introduction: escherichia coli is one of the important pathogens in human with global importance. because of the necessity for identification and the use of novel antibacterial compounds against e. coli , in this present study we focused on the antibacterial effects of synthesized thiazole, imidazole and tetrahydropyridine derivatives on e. coli .   methods : for evaluation of antibacterial eff...

background:  one of the most common infections in human is urinary tract infection  (uti)  and  uropathogenic   escherichia  coli   is  one  of  its  major causative agents . uti is extremely common among young women. children under age 5 are also highly at risk. considering the prevalence of this disease, it is necessary  to design  an appropriate  diagnostic  method  for its effective diagnos...

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

background and objective: enterohemorrhagic escherichia coli (ehec) o157:h7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (hus). cattle are an important reservoir of ehec. this research aims at vaccination with a divalent chimer protein composed of espa 120 and intimin 282 and its preventive effect of ehec o...

background and objectives: pathogenic strains of escherichia coli are a common cause of acute infectious diarrhea. the aim of this study was to investigate the frequency, virulence markers and antibiotic resistance patterns of diarrheagenic e. coli (dec) isolated from adolescents and adults in hamadan, west of iran. materials and methods: a total of 187 stool samples were collected from adults ...

reteplase is a part of tissue plasminogen activator (t-pa) used for theremoval of thrombi in blood vessels. in the present study we express the reteplase genein escherichia coli top10 and then its thrombolytic activity was measured. the recombinant plasmid pbadgiiia was transformed into the competent escherichia coli top10 and then transformed bacteria was seeded into bioreactor containing 1.5 ...

background: enterohemorrhagic escherichia coli (ehec) o157:h7 is an infectious zoonotic pathogen causing human infections. these infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. the first intimate bacterial adhesion, intimin (i), with its own receptor translocated intimin receptor (tir) and e. coli secreted protein...

abstract background: diarrhea has been recognised as a major public health problem worldwide. aprospective study was performed to determine the etiology, seasonal and age prevalence, relevant laboratory investigations, sensitivity of isolated microorganisms to current medication, and practical approaches to the diagnosis and management of diarrhea in iran, as a developing country. methods: all ...

aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...