aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...

conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

conclusions our study indicated a high frequency of cotrimoxazole resistance gene in e. coli isolates from ilam and tehran (milad) hospitals, and sul genes had a major role in cotrimoxazole resistance of these isolates. materials and methods one-hundred and forty-four e. coli isolates were identified during march 2007 to april 2012 at ilam hospitals and milad (tehran) hospital. antibiotic susce...

conclusions the β-lactamase disk test is appropriately sensitive to detect esbls in mdr isolates of e. coli. background most urinary tract infections (utis) are caused by escherichia coli (e. coli) species. due to infections outbreaks of e. coli strains with multiple mechanisms of resistance to β-lactam antibiotics, the sensitivity of confirmatory tests to detect the extended spectrum β-lactama...

background: escherichia coli is one of the most important pathogens which could be transmitted by milk. the main aim of the present study was to assess risk factors related to the occurrence of e. coli in fluid milk marketed in chittagong, bangladesh. methods: totally, 169 milk samples were collected from different entry points in chittagong, bangladesh; and also, 17 samples were directly colle...

in this study, 90 internal organ samples of poultry with symptoms of colibacillosis were obtained from maragheh poultry farms in east azerbaijan, iran. in total, 70 bacterial isolates were confirmed as escherichia coli (e. coli) strains using standard biochemical tests, and antibiotic sensitivity was determined by the disk diffusion method. antibiotics used in this study included ampicillin, pe...

objective(s): enterotoxigenic escherichia coli (etec) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. igy technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. the purpose of this study was to produce specific antibody by egg yolk antibody (igy...

background: uropathogenic escherichia coli (upec) are major bacterial agent of urinary tract infection (uti). this infection is more prevalent among women because approximately half of all women will experience a uti in their life-time and near a quarter of them will have a recurrent infection within 6–12 months. iuta protein has a major role during upec pathogenesis and consequently infection....

background and objectives: the most common serotype of enterohaemorrhagic esherichia coli group or shiga-toxin-producing e. coli is o157:h7. domestic and wild ruminants are regarded as the main natural reservoirs. o157:h7 serotype is the major cause of gastrointestinal infections in developed countries. in this study was conducted to survey on the toxigenic e. coli o157: h7 strains in milk of i...

background and objectives: auto-induction is usually employed to achieve high cell density and overproduction of proteins with a simple and low-cost operation. the efficiency of heterologous protein expression in escherichia coli is determined by different parameters. interactions between these parameters usually complicate the identification of those that contribute more to the improvement of ...