Plant materials have been found useful in decontamination of water polluted with phenolic compounds. The detoxification effect is due to peroxidases contained in plant tissue. Enzyme mediated oxidative coupling of phenol is followed by precipitation of the formed polymer and its removal from the aqueous phase. A synthetic wastewater buffered at pH = 7.4 containing 0.9 mM phenol was treated in t...

Enzymes play a central role in the detection of target molecules biotechnological fields. Most probes used are bifunctional proteins comprising enzymes and binding conjugated by chemical reactions. To create highly sensitive probe, it is essential to increase enzyme-to-binding protein ratio probe. However, if reactions required prepare probe insufficiently site-specific, may lose functionality....

Bioelectrocatalysis is a phenomenon concerned with biological catalysts, which accelerate the electrochemical reactions. Bioelectrocatalysis has been widely explored by the research community in various directions. Enzymes can catalyze different chemical reactions in living organisms by enzymes as the most important biological catalysts. These enzymatic biocatalysts are commercially available a...

amorpha-4,11-diene synthase (ads) is a key enzyme in biochemical pathway of the antimalarial agent artemisinin. an agrobacterium-mediated transformation was carried out to express a synthetic ads gene in green microalga chlamydomonas reinhardtii strain 125c with bacterial strains gv3101 and lba4404. the foreign gene was optimized based on codon usage bias of the microalga. integration of the ad...

Previously we proposed one aspect of how insects could adapt to terrestrial environments during their evolution. The hypothesis is based on a theory that have evolved an insect-specific system for cuticle formation mediated by enzyme. This enzyme, multicopper oxidase-2 (MCO2), catalyses the oxidation catecholamines using molecular oxygen as acceptor electrons from substrates. A potential advant...

Solid-state fermentation has been widely used for enzyme production. However, secreted enzymes often bind to the solid substrate preventing their detection and recovery. A series of screening studies was performed to examine the role of extraction buffer composition including NaCl, ethylene glycol, sodium acetate buffer, and Tween 80, on xylanase and cellulase recovery from switchgrass. Our res...