An extracellular xylanase from the thermophilic fungus Melanocarpus albomyces IIS 68 was evaluated for its activity and stability in the presence of polyols and salts at 60 °C, and found to be an effective protecting agent for thermal deactivation of enzyme. Response surface methodology was employed to study the synergistic effects of glycerol and NaCl (thermostabilizers) for xylanase stability...

Among biological approaches to the removal of aromatic amines and phenols from wastewater, the so-called enzyme-catalyzed polymerization and precipitation (ECPP) process relies on the use of oxidoreductases acting via radical mechanisms and characterized by a rather relaxed substrate specificity, such as laccase, tyrosinase and peroxidases. The main technical constraints of ECPP processes are d...

Deactivation of the human brain's default mode network (DMN) is regarded as suppression of endogenous activity to support exogenous task-related processes. This phenomenon has important functional relevance and insufficient DMN deactivation has been implicated in several neuropsychiatric disorders. However, the neurochemical mechanism of the DMN's deactivation remains largely unknown. In the pr...

A study was made of the coking of a commercial fresh sulfide Ni-Mo/Al2O3 catalyst in a fixed-bed reactor. The catalyst was coked using different coke precursors in the gas oil under accelerated conditions at temperatures of 400 to 450°C to yield different deactivated catalysts containing 2-20 wt% C. Two cases were studied; crushed catalyst without diffusional ...

The factors that limit the stability of adenylate kinase and acetate kinase in solution have been examined and compared with those that determine stability under conditions encountered during photochemically initiated polymer gel formation in solutions of acrylamide and N,N'-methylene-bisacrylamide. Both adenylate kinase (from rabbit and pig muscle) and acetate kinase (from E. Coli) contain cys...

Inactivation of redox enzymes and the loss enzyme’s material from its adsorbed film on electrode surface are events that have similar outputs when studied under conditions protein-film voltammetry. In current work, we present a series novel theoretical results cyclic staircase voltammetry clearly reveal how to distinguish between these two events. Theoretical model elaborated in this work compr...

N-acylethanolamine acid amidase (NAAA) is an N-terminal nucleophile (Ntn) hydrolase that catalyses the intracellular deactivation of endogenous analgesic and anti-inflammatory agent palmitoylethanolamide (PEA). NAAA inhibitors counteract this process exert marked therapeutic effects in animal models pain, inflammation neurodegeneration. While it known preferentially hydrolyses saturated fatty e...

Reduced task deactivation within regions of the default mode network (DMN) has been frequently reported in Alzheimer's disease (AD) and amnestic mild cognitive impairment (aMCI). As task deactivation reductions become increasingly used in the study of early AD states, it is important to understand their relationship to atrophy. To address this issue, the present study compared task deactivation...

Abstract Enzyme cost and stability are the main problems facing industrial applications. Consequently, Aspergillus niger NRC1ami was isolated from rotten orange recorded a promising pectinase activity (13.8 U/ml). The enzyme optimized using citrus peel pectin as sole carbon source (40 It purified by two steps purifications 632 purification folds. pure showed 14.7% carbohydrate content consists ...