a survey was carried out in seven villages around bandarabass, on persion gulf, to determine the prevalence of the intestinal protozoa infections. out of the 835 stool specimens examined, 676 samples were positive with single and multiple infections. the most prevalent of intestinal protozoa in bandarabass were entamoeba histolytica, entamoeba coli, giardia lambia and iodamoeba butschlii.

Entamoeba histolytica causes intestinal disease in endemic settings throughout the world. Diagnosis of E. histolytica infection would be improved by the identification of biomarkers that are expressed by cysts of E. histolytica, but not by cysts of closely related commensal species of Entamoeba. Herein, we describe two novel monoclonal antibodies (1A4 and 1D3) produced against a spacer region o...

INTRODUCTION Entamoeba histolytica infections were investigated in residents of the Ariquemes and Monte Negro municipalities in Rondônia State, Brazil. METHODS Stool samples of 216 individuals were processed by the spontaneous sedimentation method and analyzed by microscopy for detection of the E. histolytica/E. dispar complex, followed by the immunoassay method using an enzyme-linked immunos...

PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.

Eleitamoeba histolytica is now known to consist of two genetically distinct yet morphologically identical species, the invasive pa・rasite, retaining the name Entamoeba histolvtica Schaudinn, 1903, separating it from the noninvasive parasite, Entamoeba disPar Brumpt, 1925 [1]. In view of.this recent development in amebiasis research, there is a great need to reassess preyiously reported prevalen...

The range of clinical outcomes following Entamoeba histolytica infection is likely to be influenced by the different strains of the parasite already existing in our population. There is a need for developing faster, reliable and reproducible methods for identifying the different strains of E. histolytica. This would have a major impact on the subsequent course of treatment given to patients. In...

BACKGROUND Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to...