entamoeba gingivalis and trichomonas tenax are protozoans that live in/on the teeth, gums and sometimes tonsils, and may cause periodontitis and gingivitis. the aim of this research was studying the frequency of buccal protozoa in patients referred to dentistry clinics of jundishapur university of medical sciences. in this cross-sectional study, samples were collected from the mouth of 200 pati...

Table. Sequencing outcomes for remaining 13 of 15 samples that were negative by PCR for Entamoeba histolytica, E. dispar, and E. moshkovskii and that were not the novel species E. bangladeshi Specimen no. Sequencing outcome 1 97% identity to E. bangladeshi, but insufficient sequence length led to ambiguous assignment, conservatively removed from further analysis 2 No priming upon sequencing 3 N...

Amebiasis is caused by Entamoeba histolytica infection and can produce a broad range of clinical signs, from asymptomatic cases to patients with obvious symptoms. The current epidemiological and clinical statuses of amebiasis make it a serious public health problem worldwide. The Entamoeba life cycle consists of the trophozoite, the causative agent for amebiasis, and the cyst, the form responsi...

In recent years, substantial progress has been made in understanding the molecular and cell biology of the human parasite Entamoeba histolytica, an important pathogen with significant global impact. This review outlines some recent advances in the Entamoeba field in the last five years, focusing on areas that have not recently been discussed in detail: (i) molecular mechanisms regulating parasi...

Serum fluorescent antibody levels against Entamoeba histolytica were determined in patients with amoebic infection and in control cases. Titres of 1: 64 or above were obtained only in cases of active or recent amoebic infection, and were recorded in 75.0% of intestinal cases and in 95.2% of extraintestinal cases. Titres given by symptomless carriers of Entamoeba histolytica, though not exceedin...

Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin sugg...

A comparison of the use of three commercially available enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes to detect and differentiate Entamoeba histolytica from E. dispar was carried out. Only the Techlab kit did not cross-react with E. dispar antigens, but it was 100 times less sensitive than PCR in detection of and differentiation between the two types of Entamo...

UNLABELLED A cross-sectional molecular epidemiological study of Entamoeba species was conducted among asymptomatic Kenyan children with (n = 123) and without (n = 111) HIV infection. The prevalence of E. histolytica was low (0.4%). Entamoeba species infection was inversely related with HIV infection [HIV(+): 29.3% vs. HIV(-) 55.0%, P < 0.001]: multiple-species infection was related to higher ...

In Japan, amebiasis is domestically transmitted by two major populations: male homosexuals and mentally handicapped persons, which is remarkably different from most other developed countries where Entamoeba dispar infection is predominantly observed. Here we briefly summarize epidemiology of amebiasis in Japan. We also review our current understanding of the diversity of Entamoeba histolytica c...