Freshly isolated strains of H. pertussis show meagre growth on Bordet Gengou medium, but a series of subcultures after isolation on the medium containing decreasing amounts of blood results in an ability to grow on blood-free medium, eventually demonstrating that the organisms become to thrive on ordinary agar medium. Imamura (1952), Asano (1953) and Fukumi et al. (1953) reported that H. pertus...

employing the notions of the strong $l$-topology introduced by zhangand the $l$-frame introduced by yao  and the concept of $l$-enrichedtopological system defined in the present paper, we constructadjunctions among the categories {bf st$l$-top} of strong$l$-topological spaces, {bf s$l$-loc} of strict $l$-locales and{bf $l$-entopsys} of $l$-enriched topological systems. all of theseconcepts are ...

The effects of colony-stimulating factors (CSFs), phytohemagglutinin (PHA), and hydrocortisone on the growth of human bone marrow hematopoietic progenitor cells (granulocyte-macrophage; GM) were analyzed in a limiting-dilution assay (LDA). Both low-density bone marrow cells separated by discontinuous Percoll gradients and a T cell-depleted and progenitor-enriched cell fraction obtained by the c...

Abstract—Developing our knowledge of when pineapple roots grow can lead to improved water, fertilizer applications, and more precise culture management. This paper presents current understanding of morphological traits in pineapple roots, highlighting studies using incubation periods and various solid MS media treated with different sucrose concentrations and pH, which directly assess in vitro ...

The levels of TMC207 (R207910) that can be reached in mouse organs and the sputa of treated patients easily exceed the MIC of the compound and can therefore interfere with in vitro bacterial titrations. We studied the usefulness of protein-enriched media for the prevention of such drug carryover effects. The average MIC of Mycobacterium tuberculosis was determined on three different media: unsu...

Murine thioglycollate-elicited peritoneal macrophages were cultured in the presence of a variety of fatty acids added as complexes with bovine serum albumin. All fatty acids tested were taken up readily by the cells and both neutral and phospholipid fractions were enriched with the fatty acid provided in the medium. This generated a range of cells enriched in saturated, monounsaturated or polyu...

Oxysterols have been detected in various mammalian organs and blood. Biliary epithelium is exposed to high concentrations of cholesterol, and we have identified three keto-oxysterols (cholest-4-en-3-one, cholesta-4,6-dien-3-one, cholesta-3,5-dien-7-one) in human bile and gallstones. Because the effects of oxysterols on biliary physiology are not well defined, we investigated their biological ef...

Background: Olive pomace, as the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production.Objectives: Actinobacteria isolates were separated from an Algerian soil under olive pomace cultivation and were evaluated for their lignocellulolytic enzymes production.Materials and Methods: Isolates of Actinobacteria were separated from ...

in order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of henbane on ms medium enriched with various concentrations of kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in urom zist tak knowledge-based company plant tissue cultu...