Cell culture using a 3D method provides Various cell strategies have been developed synthetic or biological materials; most existing publications use many reagents. Bioscaffold from mouse embryonic fibroblast (MEF) enhances attachment, interaction, and production of growth factors. Since bioscaffolds could maintain stimulate pluripotency stem cells, we conducted this study to prove bioscaffold ...

Article history: Received 2 October 2017 Accepted 2 October 2017 Available online 3 October 2017 available RNA-seq dataset, they reanalyzed the expression profiles in four stages of differentiation of HESC to cardiac myocytesI) undifferentiated embryonic stem cells (ESC), II) the intermediatemesodermal stages (MES), III) cardiac precursor cells (CP) and lastly IV) cardiac myocytes (CM) (Fig. 1)...

Stem cell therapies have had tremendous potential application for many diseases in recent years. However, the tumorigenic properties of stem cells restrict their potential clinical application; therefore, strategies for reducing the tumorigenic potential of stem cells must be established prior to transplantation. We have demonstrated that syngeneic transplantation of embryonic stem cells (ESCs)...

Stem cell treatment may positively influence recovery and inflammation after shock by multiple mechanisms, including the paracrine release of protective growth factors. Embryonic stem cells (ESCs) are understudied and may have greater protective power than adult bone marrow stem cells (BMSCs). We hypothesized that ESC paracrine protective mechanisms in the heart (decreased injury by enhanced gr...

cellsderived from wharton’s jelly have been reported to display a wide multilineagedifferentiation potential, showing some similarities to both embryonic (esc)and mesenchymal stem cells (mscs). the present study investigated thedifferentiation potential of human umbilical cord mesenchymal stem cells(ucmscs) into hepatic lineage through embryonic body- like aggregates formationin the presence of...

The recent development of induced pluripotent stem cells [5] (iPSCs) and related technologies has caught the attention of scientists, activists, politicians, and ethicists alike. IPSCs gained immediate international attention for their apparent similarity to embryonic stem cells [6] after their successful creation in 2006 by Shinya Yamanaka [7] and in 2007 by James Thompson and others. Although...

Degeneration of basal forebrain cholinergic neurons (BFCNs) is associated with cognitive impairments of Alzheimer's disease (AD), implying that BFCNs hold potentials in exploring stem cell-based replacement therapy for AD. However, studies on derivation of BFCNs from embryonic stem cells (ESCs) are limited, and the application of ESC-derived BFCNs remains to be determined. Here, we report on di...

Embryonic stem cell (ESC) self-renewal and pluripotency is maintained by an external signaling pathways and intrinsic regulatory networks involving ESC-specific transcriptional complexes (mainly formed by OCT3/4, Sox2 and Nanog proteins), the Polycomb repressive complex 2 (PRC2) and DNA methylation [1-8]. Among these, Nanog represents the more ESC specific factor and its repression correlates w...

BACKGROUND Embryonic stem cells (ESC) hold great promise for the treatment of degenerative diseases. However, before clinical application of ESC in cell replacement therapy can be achieved, the safety and feasibility must be extensively tested in animal models. The common marmoset monkey (Callithrix jacchus) is a useful preclinical non-human primate model due to its physiological similarities t...