نتایج جستجو برای: embryogenic callus
تعداد نتایج: 5965 فیلتر نتایج به سال:
The present study was carried out to build up an efficient protocol for various concentrations of 2, 4D for “Callus formation and somatic embryogenesis in sugarcane (Saccharum spp L.) using various concentrations of 2, 4-D and RAPD analysis of regenerated plants in the laboratory of Biotechnology Nuclear Institute of Agriculture, Tandojam during the year 2015 to 2016. Three sugarcane varieties ...
Common bean is the most important grain legume in the human diet. Bean improvement efforts have been focused on classical breeding techniques because bean is recalcitrant to both somatic embryogenesis and in vitro regeneration. This study was undertaken to better understand the process of somatic embryogenesis in the common bean. We focused on the mechanisms by which somatic embryogenesis in pl...
The genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes are involved in embryogenesis and organogenesis in numerous species. In vitro culture of Cyclamen persicum immature ovules provides a system for investigating stem cell formation and maintenance, because lines forming either organs or ...
Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line
Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of t...
BACKGROUND Although somatic embryogenesis has an unprecedented potential for large-scale clonal propagation of conifers, the ability to efficiently induce the embryonal cultures required for somatic embryo production has long been a challenge. Furthermore, because early stage zygotic embryos remain the only responsive explants for pines, it is not possible to clone individual trees from vegetat...
The aim of this paper is to describe the evaluation of engineered herbicide-resistance in transgenic sugarcane of Herbicide treatment of crops allows economic weed control. To be useful, herbicides must distinguish between crop plant variety NCo3 10 under field conditions. and weed, and this often limits usage. Engineering herbicide resistance into crop plants via genetic transformation techniq...
Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then ...
Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then ...
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic ca...
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