an efficient method for producing doubled haploid plants of date palm ( phoenix dactylifera l.) was established using in vitro colchicine treatment of anthers. to this study male inflorescences were harvested in the appropriate developmental stage. separated anthers after one day cold (4°c) pretreatment, in uninucleate stage of microspore were transferred to the modified y 3 medium containing d...

Many of the regulatory processes occurring during plant embryogenesis are still unknown. Relatively few cells are involved, and they are embedded within maternal tissues, making this developmental phase difficult to study. Somatic embryogenesis is a more accessible system, and many important regulatory genes appear to function similar to zygotic development, making somatic embryogenesis a valua...

We developed several novel tools to genome wide screen for CNVs and SNPs in single cells. When applied to cleavage stage embryos from young fertile couples we discovered, unexpectedly, an extremely high incidence of chromosomal instability, a hallmark of tumorigenesis (Vanneste et al., Nature Medicine, 2009; Vanneste et al., Hum.Reprod., 2011). Not only mosaicisms for whole chromosome aneuploid...

Abstract Callus formation and somatic embryogenesis in potato varieties are effective ways to obtain an entire plant from a single cell. The implementation of is widely used improving materials creating new biotechnological varieties. In our research, the optimum level 1-naphthaleneacetic acid (NAA) 6-benzyl aminopurine (BAP) was determined callus tissue local Sarnav variety, Desiree variety ta...

Abstract The reaction–diffusion models can reproduce a wide variety of exquisite spatio-temporal patterns arising in embryogenesis, development and population dynamics due to the diffusion-driven (Turing) instability [102, 162].

Somatic embryogenesis could be achieved in Themeda quadrivalvis (Linn.) O. Ktze -fodder grass species on MS medium supplemented with 2,4-D. Incorporation of putrescine in the medium stimulated embryogenesis, however its lower concentration stimulated production of non-regenerative callus. Other polyamines such as spermine and spermidine could not evoke similar response. Ascorbic acid used as an...