Background: Since the freezing method was offered, much effort has been done to improve the freezing conditions in different studies and the impact of different freezing methods and mediums on oocyte, embryo and ovarian tissues has been examined. Based on studies, minerals play an important role in oocyte maturation and subsequent embryo development. Vanadium is one of the minerals, which is ve...

Current embryo vitrification methods with proven efficacy are based on the minimum volume cooling (MVC) concept by which embryos are vitrified and rewarmed ultrarapidly in a very small amount of cryopreserving solution to ensure the high viability of the embryos. However, these methods are not suitable for simultaneously vitrifying a large number of embryos. Here, we describe a novel vitrificat...

An approach to organ cryopreservation, namely, vitrification has been review ed by FAHY et al.1). RALL and FAHY2) obtained some promissing results using 8-cell mouse embryos indicating the feasibility of obtaining high survival following rapid freezing by vitrification. However, there have been only a preliminary report2) on the feasibility of obtaining offspring by the vitrification method. Th...

Vitrification is commonly used in the cryopreservation of mammalian blastocysts to overcome the temporal and spatial limitations of embryo transfer. Previous studies have shown that the implantation ability of vitrified blastocysts is impaired and that microRNAs (miRNAs) regulate the critical genes for embryo implantation. However, little information is available about the effect of vitrificati...

Very limited information on the post-implantatory effects of vitrification has been published till now. We observed in a previous study that the vitrification procedure for the cryopreservation of embryos introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. Now, we have conducted a proteomic study to determine whether protein alteration...

OBJECTIVE To assess the usefulness of the vitrification method in clinical practice. DESIGN Clinical study of vitrification of human oocytes. SETTING A university-affiliated hospital. PATIENT(S) Thirty-four patients who agreed to undergo additional IVF-ET after the failed fresh cycle using the previously vitrified/thawed oocytes. INTERVENTION(S) Surplus oocytes from the IVF-ET patients ...

The effects of vitrification on the embryo viability of the Persian sturgeon (Acipenser persicus) were investigated in this study. Vitrificant solutions (VS1-VS6) were prepared by combining propylene glycol (PG) as basic cryoprotectant plus other permeable and non-permeable cryoprotectants in different concentration. Embryos at neurulation stage were selected and incubated in six vitrificant so...

Cryopreservation of oocytes and embryos is a crucial step for widespread practical application of other techniques in domestic animal embryology. However, in contrast to the rapid development of procedures in the eighties and early nineties of the last century, new advancements with profound practical consequences have only been recently achieved. As a result of a long and controversial develop...

BACKGROUND The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000°C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of thi...