نتایج جستجو برای: duplex rt pcr
تعداد نتایج: 214870 فیلتر نتایج به سال:
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and th...
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
BACKGROUND This study aimed at investigating a potential effect caused by coccidia on the immune response to vaccine- and very virulent infectious bursal disease virus (vvIBDV) in SPF chickens. METHODS Two groups of three weeks old SPF chickens were vaccinated prior to inoculation with coccidia and challenge with virulent IBDV, all within a period of eight days. Two control groups were simila...
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with...
Gen ekspresyonunun post-transkripsiyonel düzenleyicisi olarak bilinen miRNA’lar ökaryotik canlılarda çeşitli fizyolojik ve patolojik süreçlerde rol oynamaktadır. miRNA'ların rollerinin ortaya konulması ile birlikte üzerine yapılan çalışmaların sayısı da gün geçtikçe artmaktadır. Yapılan çalışmalar bütün hücre doku tiplerinde eksprese olduğu konmuştur. miRNA ekspresyon profilinin konması için yö...
ویروس موزاییک یونجه (alfalfa mosaic virus, amv) در ایران که سبب بیماری در محصول یونجه (medicago sativa) می شود، بر اساس خصوصیات مورفولوژیکی و بیان علایم شناسایی تشخیص داده می شود. روش های متعددی جهت تشخیص این ویروس وجود دارند که می توان به آزمون های سرولوژیک و روش های مولکولی اشاره کرد. آزمون نسخه برداری معکوس تکثیر هم دمای وابسته به حلقه (revers transcription loop mediated isothermal amplifica...
materials and methods in silico analysis of the cadf gene was carried out by several software and available online tools. a duplex pcr optimized with specific primers was used for detection and differentiation of both species. to evaluate specificity and sensitivity of the test, a panel of different campylobacter spp. together with several intestinal bacterial pathogens was tested. the limit of...
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