Problem statement: The aim of this study was to find the empirical model that describes the growth kinetics of Dunaliella salina, with low production cost and to estimate parameters of this model. Approach: In this study the strain of D. salina UTEX 200 was cultivated in seawater (0.5 M NaCl) at room temperature with agitation of 150 rpm and luminous intensity of 60 mmols.m-2.s-1. The synthetic...

A fluorescent dye sensitive to membrane potential was used to follow the plasma-membrane potential in the unicellular halo-tolerant alga Dunaliella salina. The signal observed during dissipation of the plasma membrane potential by the addition of excess K(+) and valinomycin, or a protonophore, was taken as a measure of the preexisting potential. A resting potential of -85 to -100 millivolts (ne...

The green algae Dunaliella salina offers great potential for the food industry due to its high ?-carotene content. To guarantee economic profitability of cultivation, growth conditions must be improved. Therefore, effects pH and salinity on cultivation alga D. were investigated optimized. simplex method was applied find optimum these two parameters maximize biomass cell number salina. found at ...

A new hypothesis is presented for the mechanism of metabolic response during osmoregulation in the alga Dunaliella salina. We propose that the osmotic response is initiated by differential volume changes of the cytoplasm and the chloroplast (observed using the electron microscope) which alter the cytoplasmic orthophosphate concentration. This triggers a flow through the Pi/triose-phosphate shut...

A new HPLC/DAD (Diode Array Detector) method is proposed for the identification of some carotene isomers. The operating conditions adopted permit the resolution of α-carotene, all-trans-β-carotene, 9-cis-β-carotene, 13-cis-β-carotene and 15-cis-β-carotene. Moreover, the chromatographic conditions reported are simplified in respect of those reported up to now. The method is applied to the determ...