A glucose analog, 2-deoxy-d-glucose (2DG), previously shown in swine to induce many of the hallmark parameters of stress, was administered to Salmonella choleraesuis carrier-swine and the effects on Salmonella fecal shedding and tissue colonization were evaluated. Initially, pigs were divided into two groups, one that received 1 x 10 (6) S. choleraesuis and one group that received saline. At 3 ...

OBJECTIVE Micromolar concentrations of the proangiogenic metabolite deoxyribose-1-phosphate (dRP) were detected in platelet supernatants by mass spectrometry. In this study, we assessed whether the release of dRP by platelets stimulates endothelial cell migration and angiogenesis. METHODS AND RESULTS Protein-free supernatants from thrombin-stimulated platelets increased human umbilical vein e...

For recent years, disaster response management is considered as a promising field for applying methods and tools from business process management. Especially the development of adaptive workflow management systems (WfMS) brought a process-oriented management of highly dynamic disaster response processes (DRP) within tangible reach. However, time criticality, unpredictability or complex and chan...

A Cell paper by Montel-Hagen et al. (2008) published last year addresses the comparative physiology of ascorbate recycling in erythrocytes (Montel-Hagen et al., 2008). The authors claim in this paper that GLUT1-mediated glucose transport decreases whereas GLUT1-mediated dehydroascorbate (DHA) transport increases during erythropoi-esis despite markedly enhanced GLUT1 expression. To support these...

Dysregulated signaling cascades alter energy metabolism and promote cell proliferation and cyst expansion in polycystic kidney disease (PKD). Here we tested whether metabolic reprogramming towards aerobic glycolysis ("Warburg effect") plays a pathogenic role in male heterozygous Han:SPRD rats (Cy/+), a chronic progressive model of PKD. Using microarray analysis and qPCR, we found an upregulatio...

Orexin neurons are stimulated by conditions that are glucoprivic, suggesting that orexin signaling may be increased during nutritional duress. We have previously shown that injection of orexin A (OxA) into the rostral lateral hypothalamic area (rLHa) robustly and dose-dependently increases feeding behavior. Thus we hypothesized that exogenous administration of orexin A would induce a greater fe...

The present study addresses the relationship between blood flow and glucose consumption in rat primary somatosensory cortex (SI) in vivo. We examined bilateral neuronal and hemodynamic changes and 2-deoxyglucose (2DG) uptake, as measured by autoradiography, in response to unilateral forepaw stimulation. In contrast to the contralateral forepaw area, where neuronal activity, blood oxygenation/fl...

OBJECTIVE To examine the role of AMP-activated protein kinase (AMPK) in the control of glucoprivic feeding by hindbrain catecholamine neurons. RESEARCH DESIGN AND METHODS Micropunched hindbrain samples were collected from control and 2-deoxy-d-glucose (2DG)-injected rats for Western blot analysis of phosphorylated (activated) AMPK (pAMPK). Samples also were collected from 2DG-injected rats pr...

By transiently or stably overexpressing the mitochondrial fission factor dynamin-related protein-1 (Drp-1), we evaluated the role of mitochondrial division in organelle Ca2+ homeostasis and apoptotic signaling. Quantitative 3D digital microscopy revealed a split mitochondrial network in Drp-1-overexpressing cells without changes in cell viability. High-speed mitochondrial [Ca2+] ([Ca2+]m) imagi...

Authors: Grigorios Spirakis, School of Business and Economics, University of Peloponnese, Tripolis 22100, Greece; Antonios Sarantidis, Visiting Researcher, Forecasting Laboratory, Bangor Business School, Hen Coleg, College Road, Bangor, LL57 2DG, United Kingdom; Konstantinos Nikolopoulos, Bangor University, Business School, Hen Coleg, College Road, Bangor, LL57 2DG, United Kingdom, Kostas@forta...