The following method is recommended for the staining of Corynebacterium diphtheriae. It provides a good contrast between the body of the organism and the granules in it. Cover the fixed and dried film with Loeffler's methylene blue, leave for five minutes, wash with water, decolorize rapidly with 1/1000 sulfuric acid. The acid should not stay on the film for more than a few seconds, wash with w...

An improved process for slip casting ceramic articles that does not employ parting agents and affords the casting of complete, detailed, precision articles that do not possess parting lines. A wax pattern for a shell mold is provided, and an aqueous mixture of a calcium sulfate-bonded investment material is applied as a coating to the wax pattern. The coated wax pattern is then dried, followed ...

Building cost-effective models is of academic and practical value for fast measurement of soil properties, especially at a farm-scale. The aim of this study is to build quantitative models for soil total nitrogen (TN) and total carbon (TC) using visible and near infrared (VIS-NIR) spectroscopy. Dried samples (n=122) collected from an experimental farm, at Silsoe, Bedfordshire, United Kingdom, w...

A simple and efficient protocol for isolating genomic DNA from fresh and dry leaves of Vigna aconitifolia and V. trilobata was developed. It involves a modified CTAB procedure using 3% CTAB, 4% -mercaptoethanol, 2 M NaCl and 5% PVP. The extraction was carried out at 70°C. A slight increase in the concentrations of these chemical components and temperature helped in the removal of secondary meta...