Background: Lymphadenitis is the most common complication of BCG vaccination in children. Interferon-gamma (IFN-γ) plays a key role in immune response to Mycobacterial infections. In this study, the relationship of serum levels of IFN-γ and its receptor (CD119) to development of Bacillus Calmette Guerin (BCG) axillary lymphadenopathy was investigated. <em...

Peripheral blood mononuclear cells (PBMC) from 251 HIV-positive drug abusers of known clinical stage and from 40 healthy donors were tested for conventional immunologic markers (CD3, CD4, CD8, CD19, CD14, CD16/CD56, CD45 and HLA-DR). Additional cell parameters and the occurrence of spontaneous apoptosis (programmed cell death) were investigated on freshly isolated PBMC by flow cytometric measur...

Background: The ectopic expression of receptor tyrosine kinase Ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. Here we report, for the first time, expression of ROR1 gene in both tumor tissues and peripheral blood mononuclear cells (PBMC) from patients with renal cancer (RC). Methods: In the current study,...

In the search of the antigenic determinants of a 65-kDa mannoprotein (MP65) of Candida albicans, tryptic fragments of immunoaffinity-purified MP65 preparations were tested for their ability to induce lymphoproliferation of human peripheral blood mononuclear cells (PBMC). Five major peptides (T1 to T5) were shown to induce a vigorous proliferation of PBMC from the majority of the eight healthy h...

background: the ectopic expression of receptor tyrosine kinase ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. here we report, for the first time, expression of ror1 gene in both tumor tissues and peripheral blood mononuclear cells (pbmc) from patients with renal cancer (rc). methods: in the current study,...

&#x0D; Purpose: To determine the effect of fresh soy milk and its compounds (coumestrol, daidzein genistein) on apoptosis in human leukemic cells peripheral blood mononuclear (PBMC).&#x0D; Methods: The apoptotic (K562, Jurkat, U937) PBMC was determined by flow cytometry. from healthy donors were isolated conducting density gradient centrifugation principle. Lymphoprep cytotoxicity evaluated 3-(...

THIS panel was developed to quantify natural killer (NK) cell subsets in Rhesus macaques (Macaca mulatta) during SIVmac239 infection induced pathogenesis. It includes markers to monitor changes in the activation/proliferation phenotype of up to 12 NK cell populations. The performance of the staining was tested on cryopreserved lymph node samples, and on fresh and cryopreserved peripheral blood ...

Background: Dendritic cells (DCs) are the most potent stimulators of primary T cell responses and play a key role in immune reactions after stem cell transplantation. Very little is known about the cord blood (CB) dendritic cells and their potential involvement in the low incidence and lower severity of acute graft-versus-host disease after CB transplantation.   Objectives: The aim of this stud...

background: cytokines play a fundamental role in the regulation of immune responses in remission and/or relapsing of leishmaniasis. therefore, immunotherapy for the treatment of canine visceral leishmaniasis (cvl) has represented a principle approach in control of the infection. the present research aimed to evaluating the immunotherapeutic potential of a novel herbal immunomodulator drug (imod...