A dot-blot, enzyme-linked immunosorbent method and a latex agglutination test were studied for their abilities to detect Vibrio cholerae serotype O1 in aquatic samples by testing artificially contaminated water as well as samples from natural potential sources. Water samples were preenriched with alkaline peptone and then enriched with Monsur peptone water. For the dot-blot test, enriched cultu...

Objective Dot Blot (DB) assay provides highly specific results, but usually not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to be done to develop a quantitative DB assay. Material and Methods Dot blot (DB) strips for antibodies directed to human immuno...

BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. Congenital toxoplasmosis is more important during pregnancy for the first time. In this study we expressed and purified P43 Toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. METHODS The recombinant pGEMEX-1 contained Toxoplasma P43 coding...

Rice stripe virus (RSV) is a plant virus, and its infection leads to devastating disease that often causes serious yield losses in rice-producing regions. In this study, the capsid protein (CP) gene of RSV was cloned expressed E. coli (Rosetta), purified recombinant His-CP used immunize New Zealand white rabbits . After six weeks, polyclonal antibody against (PAb-CP ) obtained from serum immuni...

Virulence of the opportunistic yeast, Candida albieans, involves the interplay of many complex changes including the yeast-hyphae transition, which mainly involves protein changes. Cell wall mannoproteins are found to be the main cause of adherence of C. albieans to epithel ial cells in the first step of an infection process. In the present study, cell wall mannoproteins of intact yeast wer...

Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  Serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...

BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metaboliteproduced by Aspergillus species that contaminates a wide range ofagricultural products. OBJECTIVES: This study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of AFB1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in fo...

The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. T...