We describe here a transposon-based DNA sequencing strategy that allows the introduction of sequencing priming sites throughout a target sequence by bacterial mating. A miniplasmid was designed to select against transposon insertions into the vector. Sites of transposon insertion are mapped by the polymerase chain reaction with bacterial overnight cultures providing the templates. A small set o...

DNA sequencing is an important technology for the determination of the sequences of nucleotides that make up a given DNA fragment. In view of the limitations of current sequencing technology, it would be advantageous to have a DNA sequencing method that provides the sequences of long DNA fragments and is amenable to automation. Sequencing by Hybridization (SBH) is a challenging alternative to t...

The advent of DNA sequencing has significantly accelerated biological research and discovery. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of the human genome, in the Human Genome Project. Related projects, often by scientific collaboration across continents, have generated the complete DNA sequences of many animal, plant, ...

In this lecture, we survey a variety of different sequencing technologies, including their respective advantages and disadvantages. Then, we begin to explore the topic of genome assembly by discussing the Human Genome Project, and how genetic repeats make assembly a difficult computational task. 1 Sequencing Technologies Currently, we live in a time where genome sequencing can be done for $1,00...

Prior to the mid-1970’s no method existed by which DNA could be directly sequenced. Knowledge about gene and genome organization was based upon studies of prokaryotic organisms and the primary means of obtaining DNA sequence was so-called reverse genetics in which the amino acid sequence of the gene product of interest is back-translated into a nucleotide sequence based upon the appropriate cod...

make it much easier to obtain information on the amino acid sequence of proteins than on their structures or functions. The sequences of homologous proteins can diverge greatly over time, even though the structure or function of the proteins change little.Thus,much can be inferred about an uncharacterized protein when significant sequence similarity is detected with a wellstudied protein.This h...

The determination of the nucleotide sequence of a gene is often the ultimate goal of a research project and the frequency with which new sequences are described in the literature and computer databases is remarkable. The basic techniques that underpin these endeavours are remarkably simple and their description now frequently forms part of the very earliest portion of a biological science under...

Background: β-thalassemia is one of the most widespread disease in the world, including Iran. In this study, we reported, for the first time, A 290-bp β-globin gene deletion in the south of Iran. Methods: Four individuals from three unrelated families with Arabic ethnic background were studied in Khuzestan Province. Red blood cell indices and hemoglobin analysis were carried out accor...