In order to establish the most sensitive and specific method in the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense , mice and patients, a comparative study on DNA extraction, PCR, hybridization and PCR-hybridization was conducted. The results showed that the sensitivity of PCR-hybridization was 10 times higher than that of PCR and 1000 times higher than that of hybridization....

Theoretical models of localized DNA reactions on platforms indicate potential benefits to conventional DNA hybridization reactions. Recently locality has been proposed as a novel approach to speed up DNA hybridization reactions as well as to minimize incorrect binding among DNA sequences. Here we experimentally report evidence for a 169-fold speedup of localized DNA hybridization chain reaction...