In order to establish the most sensitive and specific method in the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense , mice and patients, a comparative study on DNA extraction, PCR, hybridization and PCR-hybridization was conducted. The results showed that the sensitivity of PCR-hybridization was 10 times higher than that of PCR and 1000 times higher than that of hybridization....
