In many crop species, DNA fingerprinting is required for the precise identification of cultivars to protect the rights of breeders. Many families of retrotransposons have multiple copies throughout the eukaryotic genome and their integrated copies are inherited genetically. Thus, their insertion polymorphisms among cultivars are useful for DNA fingerprinting. In this study, we conducted a DNA f...

Melon, Cucumis melo L. is an important vegetable crop worldwide. At present, there are phenomena of homonyms and synonyms present in the melon seed markets of China, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. Molecular markers, especially microsatellites or simple sequence repeats (SSRs) are playing increasin...

In spite of the increasing application of DNA fingerprinting to natural populations and to the genetic identification of humans, explicit methods for estimation of basic population genetic parameters from DNA fingerprinting data have not been developed. Contributing to this omission is the inability to determine, for multilocus fingerprinting probes, relatively important genetic information, su...

The expediency of using unique SSR DNA-markers Schizophyllum commune for population genetic assays in various fungal species has been demonstrated. In Auricularia auricula-judae and Irpex lacteus, we observed formation heterogeneous amplicons mostly up to 500 bp length that ensured their high resolution facilitated data analysis. established sets molecular markers are efficient DNA-fingerprinti...

Random amplified polymorphic DNA (RAPD) PCR is a feasible method to evaluate genotoxin-induced DNA damage and mutations. In this study, Lactobacillus plantarum ATCC 14917T, Enterococcus faecium DSMZ 20477T, Escherichia coli PQ37 and Saccharomyces cerevisiae S441 were screened for DNA genetic alterations by DNA fingerprinting using M13 and LA1 primers after treatment with three compounds forming...

In this article, we discuss the implications of a recent European Court of Human Rights (ECHR) decision about the use of retained DNA profiles in criminal cases. Met with polar but equal passion from both the privacy lobby and law enforcement, this case has opened concerns regarding ethics in the nascent science of DNA profiling. Although the technology is touted as the most exciting breakthrou...

"Hands on" laboratory exercises are excellent teaching tools because they have the potential to reach students with all types of learning styles. Unfortunately the large monetary investment required to obtain supplies and equipment and train instructors prevents many schools from providing hands-on learning experiences with DNA technology. Students in non-laboratory courses are even less likely...

Repetitive sequence-based polymerase chain reaction fingerprinting was used to characterize 23 vancomycin-nonsusceptible enterococcal isolates from 2003 to 2004. Five genetically related clusters spanned geographically distinct referring centers. DNA fingerprinting showed infant-to-infant transmission from referring institutions. Thus, community healthcare facilities are a source of vancomycin-...

Some methods of statistical analysis of data on DNA fingerprinting suffer serious weaknesses. Unlinked Mendelizing loci that are at linkage equilibrium in subpopulations may be statistically associated, not statistically independent, in the population as a whole if there is heterogeneity in gene frequencies between subpopulations. In the populations where DNA fingerprinting is used for forensic...