methylated and un-methylated control dna is an important part of dna methylation studies. although human and mouse dna methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. in this paper a simple method of generating methylated and un-methylated control dna is described. whole...

The combination of DNA and π-conjugated polyelectrolytes (CPEs) represents a promising approach to develop DNA hybridization biosensors, with potential applications for instance in the detection of DNA lesions and single-nucleotide polymorphisms. Here we exploit the remarkable optical properties of a cationic poly[3-(6'-(trimethylphosphonium)hexyl)thiophene-2,5-diyl] (CPT) to decipher the self-...

Aspartic proteases are a relatively small group of enzymes which express in various nematodes including Onchocerca volvulus. An estimation of the gene copy number corresponding to the OV7A clone, which contains a cDNA insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of O. volvulus, was made by slot blot analysis in a closely related species O. gibsonig...

introduction: certain types of human papillomavrus (hpv) are associated with cervical intraepithelial neoplasia (cin) and squamous cell carcinoma (scc). the aim of theobservations reported here was to determine whether the prognosis for invasive cancers of the uterine cervix is related to the type of human papillomavirus asociated with the tumor. material and methods: twenty patients with invas...

Single-copy nuclear DNAs (scnDNAs) of eight species of arvicoline and six species of murine rodents were compared using DNA-DNA hybridization. The branching pattern derived from the DNA comparisons is congruent with the fossil evidence and supported by comparative biochemical, chromosomal, and morphological studies. The recently improved fossil record for these lineages provides seven approxima...

Abstract The development of approaches for predictive calculation hybridization properties various nucleic acid (NA) derivatives is the basis rational design NA-based constructs. Modern advances in computer modeling methods provide feasibility these calculations. We have analyzed possibility calculating energy DNA/RNA and RNA/RNA duplex formation using representative sets complexes (65 75 compl...

to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of th...

medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. these compounds especially antioxidants can increase oxidation of dna in response to wounds. nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...

Seventy-eight aerotolerant Campylobacter isolates were characterized phenotypically and by DNA hybridization (hydroxyapatite method at 50 and 65 degrees C). Two DNA relatedness groups were found. (i) Sixty-four strains belonged to aerotolerant Campylobacter DNA hybridization group 2. These organisms were isolated from humans, primarily with diarrheal illness, and animals on several continents. ...