Background: It was previously shown that the activity of a serine protease from a moderately halotolerant Bacillus aquimaris VITP4 strain is active in a wide range of pH and temperatures and could be modulated by the presence of the divalent metal ions.  Objectives: In the present study, a quantitative analysis was done in order to explore the parameters that are contributing to the protease a...

Biological membrane fusion employs divalent cations as protein cofactors or as signaling ligands. For example, Mg2+ is a cofactor for the N-ethylmaleimide-sensitive factor (NSF) ATPase, and the Ca2+ signal from neuronal membrane depolarization is required for synaptotagmin activation. Divalent cations also regulate liposome fusion, but the role of such ion interactions with lipid bilayers in Ra...

The enzymatic activity of citrate synthase from pig heart can be inhibited by various ions. The order of inhibitory effect of monovalent cations is Li+ > K+ > Na+ > Cs+ and that of anions is SCN> Cl> tartrate. Divalent cations such as Ca2+ and Mg2+ are much more effective than monovalent cations, but thiocyanate is as effective as divalent cations. ATP is also a potent inhibitor, but dATP has n...

The ionophoretic antibiotics, monensin and lasalocid, bind numerous mono- and divalent cations, primarily in dimeric complexes that facilitate the passage of metal ions through hydrophobic lipid membranes. Ionophores vary in their affinity for metal ions. Similarly, transport rates of the resulting complexes depend in part upon the affinity constant of the drug ion-binding interaction, and in p...

The effects of monovalent, divalent, and trivalent cations on the binding of tetrodotoxin and saxitoxin to intact nerves and to a preparation of solubilized nerve membranes have been examined. All eight divalent and trivalent cations tested, and the monovalent ions Li(+), Tl(+), and H(+) appear to compete reversibly with the toxins for their binding site. The ability of lithium to reduce toxin ...

SalNa (sodium salinomycin) reacts with divalent transition metal ions of Co(II), Ni(II), Cu(II) and Zn(II) to produce novel compounds characterized by various spectroscopic methods. The interaction of metal (II) ions with SalNa results in the formation of mononuclear complexes of a general composition of [M(Sal)2·(H2O)2] nH2O (n = 0 or 2) where the divalent cations replace Na ions from the cavi...