For the determination of the phosphatase content of yeast many methods have been used (l-3). The usual method is to hydrolyze a glycerophosphate and measure the amount of inorganic phosphate liberated. King and Armstrong (4) have shown, however, that phenyl phosphate could be used as a substrate and the phenol det,ermined. To be satisfactory the method chosen must give a measurable amount of hy...

Nitrite and nitrate are main stable products of nitric oxide, a pivotal cellular signaling molecule, in biological fluids. Therefore, accurate measurement of the two ions is profoundly important. Nitrite is difficult to be determined for a larger number of interferences and unstable in the presence of oxygen. In this paper, a simple, cost-effective and accurate HPLC method for the determination...

Recent research has focused on the crystal growth of strontium phosphates via different methods due to the bioactivity and biocompatibility of these materials with bone tissue. Here, we use a biomimetic method to synthesize strontium hydrogen phosphate/gelatin composites via single diffusion in gelatin. We compare the composite crystals with analytical-grade strontium hydrogen phosphate using i...

an efficient and eco-friendly protocol for the preparation of 2-amino-4h-chromenes employing a multi-component, one-pot condensation reaction between aromatic aldehydes, malononitrile and1-naphthol has been developed. the reaction of 1-naphthol with malononitrile and various aromatic aldehydes was carried out in water-ethanol (1:1) at reflux using 20 mol% of diammonium hydrogen phosphate as cat...

Combretastatin A4 disodium phosphate (CA4DP) is a prodrug of combretastatin (CA4), microtubule-disassembling agent that exhibits antitumor effects by inhibiting tumor cell proliferation and inducing morphological changes apoptosis in vascular endothelial cells tumors. However, cardiotoxicity induced ischemia hypertension severe adverse event. In this study, we focused on the fact phosphodiester...

The structure of synthetic disodium magnesium disulfate decahydrate at 180 K consists of alternating layers of water-coordinated [Mg(H(2)O)(6)](2+) octahedra and [Na(2)(SO(4))(2)(H(2)O)(4)](2-) sheets, parallel to [100]. The [Mg(H(2)O)(6)](2+) octahedra are joined to one another by a single hydrogen bond, the other hydrogen bonds being involved in inter-layer linkage. The Mg(2+) cation occupies...

In other papers (Lyon, 1923-24, 1927) results have been reported which appeared to show that neutral solutions of sodium or potassium phosphate serve to catalyze the enzymatic production of CO2 by plant tissues. The oxidising enzymes involved were those of Elodea canadensis, wheat seedlings, and potato tubers, the latter studied only in aqueous extracts. We shall now present additional proof of...

Phosphate limitation is an important environmental stress that affects the metabolism of various organisms and, in particular, can trigger the virulence of numerous bacterial pathogens. Clostridium perfringens, a human pathogen, is one of the most common causes of enteritis necroticans, gas gangrene and food poisoning. Here, we focused on the high affinity phosphate-binding protein (PBP-1) of a...