Turnover of actin filaments in cells requires rapid actin disassembly in a cytoplasmic environment that thermodynamically favors assembly because of high concentrations of polymerizable monomers. We here image the disassembly of single actin filaments by cofilin, coronin, and actin-interacting protein 1, a purified protein system that reconstitutes rapid, monomer-insensitive disassembly (Briehe...

Disassembly of the nuclear lamina is a key step during open mitosis in higher eukaryotes. The activity of several kinases, including CDK1 (cyclin-dependent kinase 1) and protein kinase C (PKC), has been shown to trigger mitotic lamin disassembly, yet their precise contributions are unclear. In this study, we develop a quantitative imaging assay to study mitotic lamin B1 disassembly in living ce...

Several strategies have been detected in the extant literature to understand how Circular Economy (CE) can be pursued. Considering all End-of-Life (EoL) management practices detected, disassembly processes identified as strategic. However, only scattered attempts explored digital technologies (specifically, simulation) support CE adoption, by focusing on processes. This research detects, throug...

Although Snail is essential for disassembly of adherens junctions during epithelial-mesenchymal transitions (EMTs), loss of adherens junctions in Drosophila melanogaster gastrula is delayed until mesoderm is internalized, despite the early expression of Snail in that primordium. By combining live imaging and quantitative image analysis, we track the behavior of E-cadherin-rich junction clusters...

Sublethal renal ischemia induces tubular epithelium damage and kidney dysfunction. Using NRK-52E rat proximal tubular epithelial cells, we have established an in vitro model, which includes oxygen and nutrients deprivation, to study the proximal epithelial cell response to ischemia. By means of this system, we demonstrate that confluent NRK-52E cells lose monolayer integrity and detach from col...

In this paper, a bi-objective mixed-integer linear optimization model for Closed-loop Supply Chain Network Design Problem (CLSCND) is developed. The proposed model includes both the forward and reverse directions and includes different types of facilities, namely, manufacturing/remanufacturing centers, warehouses, and disassembly centers. The first objective function tried to minimize the total...

Environmental protection legislation, consumer interest in “green” products, a trend toward corporate responsibility and recognition of the potential profitability of salvaging operations, has resulted in increased interest in product take back. However, the cost effectiveness of product take-back operations is hampered by many factors, including the high cost of disassembly and a widely varyin...