Dal5p has been shown previously to act as an allantoate/ureidosuccinate permease and to play a role in the utilization of certain dipeptides as a nitrogen source in Saccharomyces cerevisiae. Here, we provide direct evidence that dipeptides are transported by Dal5p, although the affinity of Dal5p for allantoate and ureidosuccinate is higher than that for dipeptides. Allantoate, ureidosuccinate, ...

Abstract As low‐molecular‐weight hydrogelators, dipeptide hydrogel materials are suited for embedding multiple organic molecules and inorganic nanoparticles. Herein, a simple but precisely controllable method is presented that enables the fabrication of dipeptide‐based hydrogels by supramolecular assembly inside microfluidic channels. Water‐soluble quantum dots (QDs) as well premixed porphyrins...

Dipeptide monoester prodrugs of floxuridine were synthesized, and their chemical stability in buffers, resistance to glycosidic bond metabolism, affinity for PEPT1, enzymatic activation and permeability in cancer cells were determined and compared to those of mono amino acid monoester floxuridine prodrugs. Prodrugs containing glycyl moieties were the least stable in pH 7.4 buffer ( t 1/2 < 100 ...

A dipeptide hydrolase from the brush border of guinea-pig intestinal mucosa was purified. The enzyme resembles another dipeptide hydrolase isolated from the cytosol fraction of intestinal mucosa. Studies on the binding of cytosol peptide hydrolase to brush-border membranes indicate that the enzyme found in the brush border may be a cytoplasmic contaminant.

Transport of labeled glycyl-L-proline has been shown to occur with highly purified brush border membrane vesicles from the epithelial cells of rabbit small intestine and renal cortex. With 1-min incubation, transport occurs mainly as the intact dipeptide since less than 10% of the dipeptide in the medium is hydrolyzed within the period. The properties of the dipeptide transport system are simil...

We present the resonant two-photon ionization (R2PI) spectra as well as the UV–UV and IR–UV double resonance spectra for the cyclic dipeptide Phe-Ser. The R2PI spectrum shows five strong transitions in the region of 37 500–37 900 cm . By performing UV–UV double resonance spectroscopy, we distinguished 5 different conformers. For each of these conformers, the origin is the most intense transitio...

The growing string method is a powerful tool in the systematic study of chemical reactions with theoretical methods which allows for the rapid identification of transition states connecting known reactant and product structures. However, the efficiency of this method is heavily influenced by the choice of interpolation scheme when adding new nodes to the string during optimization. In particula...

Conventional two-dimensional culture has greatly assisted tissue engineering research but the absence of tissue-like architectures and availability unidirectional cell growth limited its use in biomedical applications. Hydrogels, particularly peptide-based, due to their solid-like porous structures, high water content, biocompatibility offer three-dimensional microenvironments for help mimic vi...

Small and large molecules may localize their energy in specific bonds or generally in vibrational modes for extended periods of time, an effect which may have dramatic consequences in reaction dynamics. Periodic orbits offer the means to identify phase space regions with localized motions. The author demonstrate that techniques to locate periodic orbits developed for small molecules can be appl...