18O labeling studies on the catechol 1,2-dioxygenase-catalyzed oxidative cleavage of pyrogallol demonstrate that the enzyme functions both as a dioxygenase and a monooxygenase in this reaction. Two products are observed, 2-pyrone-6-carboxylic acid, 99% singly labeled at the carboxylate, and 2-hydroxy-cis,cis-muconic acid, 74% doubly labeled (one 18O at each carboxylate) and 24% single labeled (...

Comamonas testosteroni YZW-D was isolated from Passaic River sediment for its ability to degrade isophthalate and terephthalate. Degradation of the two isomeric compounds proceeds via separately inducible catabolic pathways that converge at protocatechuate. Analysis of the catabolic pathways by which these two isomers are degraded demonstrated that a cis-dihydrodiol intermediate is involved in ...

Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions challenging due to the harsh environmental conditions. To date, there little information available concerning capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding degradative enzymes identified using who...

One strain of bacteria, designated NCE312, was isolated from a naphthalene-digesting chemostat culture that was inoculated with creosote-contaminated marine sediment. The strain was isolated based on its ability to grow using naphthalene as a sole carbon source. In addition, the strain degraded 2-methylnaphthalene and 1-methylnaphthalene. Analysis of a 16S rRNA gene sequence from NCE312 placed ...

In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran degrader Terrabacter sp. strain DBF63, were identified. When overexpressed in E...

A novel bacterial strain was isolated from industrially contaminated waste water. In the presence of crude oil, this shown to reduce rate total petroleum hydrocarbons (TPH) up 97.10% in 24 h. This bacterium subsequently identified by 16S rRNA gene sequence analysis and affiliated Serratia genus RDP classifier. Its genome sequenced annotated, genes coding for catechol 1,2 dioxygenase naphthalene...

A Gram-negative bacterium, designated as strain KB2, was isolated from activated sludge and was found to utilize different aromatic substrates as sole carbon and energy source. On the basis of morphological and physiochemical characteristics and 16S rRNA gene sequence analysis, the isolated strain KB2 was identified as Stenotrophomonas maltophilia. Strain KB2 is from among different Stenotropho...