The acyl-CoA:1,2-diacylglycerol acyltransferase (EC 2.3.1.20) activity of isolated fat cells was predominantly (89%) localized to the microsomal subcellular fraction by assays based on the conversion of 1,2-[3H]diacyl-sn-glycerol to triacylglycerol using 1 to 4 mug of protein. A complementary assay based on the conversion of [3H]palmitoyl-CoA to triacylglycerol was developed. These methods, 100...

We expressed the g -subspecies of protein kinase C ( g -PKC) fused with green fluorescent protein (GFP) in various cell lines and observed the movement of this fusion protein in living cells under a confocal laser scanning fluorescent microscope. g -PKC–GFP fusion protein had enzymological properties very similar to that of native g -PKC. The fluorescence of g -PKC– GFP was observed throughout ...

Severe scaly lesions in the skin, especially in the were accompanied by growth retardation, impaired feet and tail, of the rat were induced by feeding a diet deficient fertility, increased water consumption, and diminished in essential fatty acids (EFA). Analysis of the fatty acids in skin urine production^ B~~~ and B~~~ proposed that linoleic lipids of these EFA-deficient rats showed a marked ...

We expressed the gamma-subspecies of protein kinase C (gamma-PKC) fused with green fluorescent protein (GFP) in various cell lines and observed the movement of this fusion protein in living cells under a confocal laser scanning fluorescent microscope. gamma-PKC-GFP fusion protein had enzymological properties very similar to that of native gamma-PKC. The fluorescence of gamma-PKC- GFP was observ...