Food grade pectinases from Aspergillus niger are predominantly used as processing aids in industries. In the present investigation, efforts towards standardization for pilot scale production of pectinase enzyme from A. niger were made. The crude enzyme was studied for its kinetics to optimize substrate concentration and processing parameters on the basis of polygalacturonic activity. Km (Michae...

In the present work, optimization of pectinase-assisted and tri-solvent-mediated extraction of lycopene from waste tomato peels was carried out. The optimized parameters for enzymatic pre-treatment were 2% pectinase concentration, pH 5.5, 4-h incubation, 45 °C and 150 rpm. Maximum recovery of lycopene from tomato peels using optimized tri-solvent extraction was achieved at 45 °C, 120-min incuba...

Cold–active polygalacturonase and amylase producers were screened using enrichment culture technique. The diverse sources screened were cold stored spoilt fruits and vegetables from different local super markets, market waste dumped soils, fruit waste dumped soils, mountain soils and Himalayan soils. About sixty yeasts showing pectinolytic activity were isolated by ruthenium red plate assay. Ei...

Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and th...

a new dimeric orthopalladated complex was synthesized via reaction of 2,3-dimethoxy benzaldehyde oxime with palladium chloride and lithium chloride in methanol as solvent and sodium acetate as base at room temperature. the catalytic activity of this dimeric [pd{c6h2(-ch=noh)-(ome)2-2,3}(µ-cl)]2 complex as an efficient, air, and moisture tolerant catalyst was investigated in mizoroki–heck cross ...

An effective statistical tool for increasing and boosting the production of xylanase pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be response surface methodology (RSM) using central composite design (CCD). Optimum was achieved under conditions a temperature 28 °C, pH 8.38, inoculum size 4% (w/v) agitation speed 94 rpm 48 h. The experimental respons...

Enzymes are bio-active compounds or catalyst that regulates many chemical reactions in living tissues and cells (Prathyusha and Suneetha, 2011). These enzymes have useful applications in paper, fruit and textile industries. Almost 75% of the estimated sale value among industrial enzymes in 1995 has been contributed by pectinases. As a result, Pectinase became one of the futuristic useful enzyme...