Hemoglobin contamination in antiserum occurs frequently in immunohematology. The hemoglobin obscures the ring test for the observation of antigen-antibody reaction. A simple method to remove hemoglobin from antiserum is described in this paper. An appropriate amount of CM-Sephadex was added to antiserum containing hemoglobin and the mixture was packed into a cellulose tube, followed by dialysin...

The purpose of this investigation was to examine the in vitro susceptibility of Candida albicans to a mixture of chlorhexidine and calcium hydroxide using a tube dilution test. A suspension containing C. albicans was exposed to 2% chlorhexidine solution, calcium hydroxide paste and 2% chlorhexidine mixed with calcium hydroxide in plastic tissue culture wells. The tested agents were incubated wi...

The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the ...

Essential oils from Ferulago asparagifolia Boiss., F. galbanifera (Miller) W. Koch, F. humilis Boiss. (Endemic), F. trachycarpa Boiss. growing in Turkey were evaluated against 15 microorganisms for their antifungal and antibacterial activity using an agar tube dilution and microdilution broth susceptibility assay, respectively. The essential oil compositions were investigated by GC/MS. Inhibito...

The purpose of this investigation was to study, in vitro, the antifungal effect of mineral trioxide aggregate (MTA) using a tube-dilution test. MTA was tested freshly mixed and after 24 h set on Candida albicans. The tested MTA was incubated with C. albicans for 1 h, 24 h, and 3 days. Results showed that the freshly mixed MTA was effective in killing the tested fungi after 1 day of contact, whe...

A project was initiated to investigate concerns regarding the application of duct smoke detectors (ASHRAE RP-1079). The objective of the research was to investigate the effects of driving forces, dilution, aging, smoke stratification, filters, and sample tube efficacy on smoke detector response. As one part of this project, a series of full-scale fire tests were conducted in a ten-story facilit...