An NADP-dependent methylene tetrahydromethanopterin (H4MPT) dehydrogenase has recently been proposed to be involved in formaldehyde oxidation to CO2 in Methylobacterium extorquens AM1. We report here on the purification of this novel enzyme to apparent homogeneity. Via the N-terminal amino acid sequence, it was identified to be the mtdA gene product. The purified enzyme catalyzed the dehydrogen...

In this work we investigated the intracluster ion molecule reactions following the generation of Mg(+) within the polar clusters (water, methanol, ether and acetonitrile), using time of flight mass spectrometry. In the case of Mg(+)/water and Mg(+)/methanol, dehydrogenation reactions are observed after the addition of five molecules. However, no dehydrogenation reactions are observed in the cas...

The dehydrogenation reaction of cholest-7-en-3P-ol (I) to cholesta-5,7-diem3/3-ol (II) in the presence of NADH was studied in rat liver microsomes and in microsomal acetone powder preparations, using [3Lu-“Hlcholest-‘7-en-3fi-o1. It was found that the reaction was inhibited by menadione, adenosine diphosphate, potassium ferricyanide, and cytochrome c while p-cresol had no effect. These results ...

Nanocrystalline magnesium hydride powder was synthesized by mechanical milling of MgH2 in a planetary ball mill for various times. The effect of MgH2 structure, i.e. crystallite size, lattice strain, particle size and specific surface area on the hydrogen desorption properties was investigated. A single peak of hydrogen desorption was observed for as-received powder, exhibiting an average parti...

In this study the catalytic dehydrogenation of ethylbenzene to styrene was investigated in a simulated tubular sodalite membrane reactor. The high quality microporous sodalite membrane was synthesized by direct hydrothermal method and characterized by single gas permeation measurements. The performance of the prepared membrane showed high potential for application in a dehydrogenation membrane ...

Ezlopitant is metabolized by cytochrome P450 primarily to two metabolites: a benzyl alcohol and a corresponding alkene. The alkene arises as a direct product of metabolism of ezlopitant rather than through dehydration of the benzyl alcohol. The mechanism of this cytochrome P450 (P450)-catalyzed dehydrogenation reaction was probed with five different deuterium-labeled analogs of ezlopitant. At s...

An electrophilic substitution (SE) reaction of BN isosteres has been investigated for the dehydrogenation of ammonia borane (AB) by metal chlorides (MCl2) using various ab initio calculations. In contrast to the typical SE reaction occurring at the carbon atom, the nitrogen atom in AB serves as the reaction center for the SE reaction with the boron moiety as the leaving group when the MCl2 appr...

The effects of NbN nanoparticles synthesized via a simple "urea glass" route on the dehydrogenation properties of LiAlH4 have been systematically investigated. The particle size of the as-synthesized NbN nanoparticles is determined to be about 10 nm. The surface configuration and dehydrogenation behaviors of the 2 mol% NbN-doped LiAlH4 (2% NbN-LiAlH4) system are also discussed. It is found that...

The dehydrogenation enthalpies of Ca(AlH(4))(2), CaAlH(5), and CaH(2)+6LiBH(4) have been calculated using density functional theory calculations at the generalized gradient approximation level. Harmonic phonon zero point energy (ZPE) corrections have been included using Parlinski's direct method. The dehydrogenation of Ca(AlH(4))(2) is exothermic, indicating a metastable hydride. Calculations f...

Using density functional theory we examine the crystal structure and the finite-temperature thermodynamics of formation and dehydrogenation for the quaternary hydride Li4BN3H10. Two recent studies based on x-ray and neutron diffraction have reported three bcc crystal structures for this phase. While these structures possess identical space groups and similar lattice constants, internal coordina...