Recent progress in the development and production of new, innovative protein therapeutics require rapid adjustable high-resolution bioseparation techniques. Sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using a borate (B) cross-linked dextran (D) separation matrix is widely employed today for consistency analysis therapeutic proteins manufacturing release testing. Transient cro...

BACKGROUND Although increased capillary permeability is the major clinical feature associated with severe dengue infections the mechanisms underlying this phenomenon remain unclear. Dextran clearance methodology has been used to investigate the molecular sieving properties of the microvasculature in clinical situations associated with altered permeability, including during pregnancy and in vari...

Ten patients with active rheumatoid arthritis were given a total dose infusion of iron dextran for anaemia. One had an immediate anaphylactoid reaction; the other nine had a brief exacerbation of arthralgia and joint dysfunction of up to seven days' duration. The erythrocyte sedimentation rate rose by an average of 23 mm. in one hour (Westergren), but this often took longer than seven days to s...

The monoclonal antibody (MCA) SucroTest is a commercially available kit (Midland Research Laboratories Inc) for the specific analysis of dextran in juices and sugar. The method is simple and quick and is based on the selective and specific reaction between the antibody and its antigen (in this case dextran). The resultant insoluble complex causes turbidity which is measured after a set time usi...

Cationic polysaccharides were synthesized by conjugation of various monoquaternary (MQ) ammonium oligoamines to oxidized dextran by reductive amination and tested for gene transfection. Polycations of dextran grafted with MQ ammonium oligoamines of two to four amino groups were investigated for their ability to cause pCMV-GFP encoding for green fluorescence protein and beta-Gal encoding for bet...

A decreased A/G ratio may indicate multiple myeloma, autoimmune disease, cirrhosis, or kidney disease. The patient’s electrophoretogram was normal, and subsequent immunofixation was negative, which ruled out monoclonal gammopathy. The SPE (Fig. 1) showed an albumin of 64.4% and normal A/G ratio, which suggests spurious hyperproteinemia. The patient was being treated with dextran (1 ). In the as...

To elucidate the mechanism by which activation of the contact system of blood coagulation leads to expression of fibrinolytic activity, we have determined the molecular characteristics of the plasminogen activators present in dextran sulfate-treated euglobulin fractions by electrophoretic-zymographic analysis and specific immunoadsorption. In addition to free and protease inhibitor-bound tissue...

To date, gold nanoparticles (AuNPs) have been investigated for diverse bioapplications. Generally, AuNPs are engineered to possess surface coating with organic/inorganic shells to increase colloidal stability in biological solutions and to facilitate chemical conjugation. In the present study, we developed a strategy to prepare dextran-coated AuNPs with control over its size by simply boiling a...

Poly-d-lysine of high molecular weight enhances interferon induction in mice by the double-stranded complex of polyinosinic and polycytidylic acids and is superior to diethylaminoethyl-dextran in this respect.