This work presents UV first derivative spectrophotometry as a precise, accurate, and feasible method for simultaneous determination of diminazene diaceturate and phenazone in bulk and dosage forms. The absorbance values of diminazene diaceturate and phenazone aqueous mixture were obtained at 398 nm and 273 nm, respectively. The developed method was proved to be linear over the concentration ran...

Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (s...

Article history: Received on: 15/10/2012 Revised on: 06/12/2012 Accepted on: 08/01/2013 Available online: 28/01/2013 In this study zero, first and second order derivative spectrophotometric method were developed for the estimation of sitagliptine. In zero order spectrophotometery, absorbance value was measured at 267nm. In first derivative spectrophotometry amplitudes were measured at 213nm. In...

Losartan, a highly effective blood pressure lowering agent, has been widely used for the treatment of hypertension. A fast and reliable method for the determination of losartan was highly desirable to support formulation screening and quality control. A first derivative UV spectroscopic method was developed for determination of losartan in the tablet dosage form. The first derivative spectrum r...

Three methods are presented for simultaneous determination of ibuprofen and paracetamol without previous separation. The first method depends on first derivative UV spectrophotometry, with zero-crossing measurement. The first derivative amplitudes at 230 and 290 nm were selected for the assay of ibuprofen and paracetamol, respectively. The second method depends on first derivative of the ratio-...

The methods for quantitative determination of meloxicam and mefenamic acid in pharmaceuticals by classic spectrophotometry - zero order derivative, first and second order derivatives spectrophotometry is described, using "peak - peak" (P-P) and "peak - zero" (P-O) measurements. The calibration curves are linear within the concentration range of 4.0 - 14.0 microg/mL for meloxicam and 14.0 - 24.0...

Ricin is a toxic lectin which presents a potential security threat. Its rapid detection is highly desirable. Here we present a colorimetric bioassay based on the aggregation of carbohydrate-stabilised gold nanoparticles which has been used to detect Ricinus communis Agglutinin 120 (RCA(120)) - a ricin surrogate. To achieve a stable and robust sensing system the anchor chain length and the densi...

Ultraviolet UV absorbance measurements are subject to significant error using a standard spectrophotometer when particles or aggregates that scatter light are present. True UV absorbance for highly turbid waters should be measured using integrating sphere IS spectrophotometry that allows the collection of reflected and transmitted radiation simultaneously. This is especially important when the ...

Abstract: Background: An anti-diabetic formulation consisting of vildagliptin and remogliflozin was prescribed for better glycemic control. In the present study a simple, rapid derivative spectrophotometric methods were evolved to analyze these two analytes from formulations. Methods: Two processed UV established by measuring peak amplitude at zero-crossing second spectra analytes. The procedur...