نتایج جستجو برای: depc
تعداد نتایج: 120 فیلتر نتایج به سال:
Very reliable methods have been developed to extract RNA from different tissues and cell lines (1, 2, 3). RNA preparation, however, is still cumbersome and time consuming, especially when extracting a large number of cells (>10) and cell samples. The isolation of RNA from leucocytic cells is particularly difficult for three reasons: a) high levels of endogenous RNase; b) low amount of RNA (RNA/...
Telomeres, the ends of chromosomes, are composed of many kilobases of TTAGGG repeats in vertebrates. These repeats are added by the enzyme telomerase, a ribonucleoprotein DNA polymerase that synthesizes the repeats based on its RNA template. A sensitive assay combining telomerase activity and the polymerase chain reaction (PCR) known as the telomeric repeat amplification protocol (TRAP) has bee...
List of Nonstandard Abbreviations: UGT, UDP-glucuronosyltransferase; M3G, morphine-3-ß-glucuronide: M6G, morphine-6-ß-glucuronide; DEPC, diethyl pyrocarbonate; PCR, polymerase chain reaction; HEK293, human embryonic kidney 293; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; HPLC, high performance liquid chromatography; N-glycosylation, asparagine-linked glycosylation. ABSTRA...
The ubiquitous octamer-binding protein oct-1 contains a POU domain required for DNA binding, which can be subdivided into a POU-specific domain and a POU homeo domain. We have overproduced the POU domain and the POU homeo domain in a vaccinia expression system, purified both polypeptides to near homogeneity, and compared their DNA-binding properties. In contrast to the POU domain, the homeo dom...
Alpha-glucosidase I initiates the trimming of newly assembled N-linked glycoproteins in the lumen of the endoplasmic reticulum (ER). Site-specific chemical modification of the soluble alpha-glucosidase I from yeast using diethylpyrocarbonate (DEPC) and tetranitromethane (TNM) revealed that histidine and tyrosine are involved in the catalytic activity of the enzyme, as these residues could be pr...
We have designed a dodecamer d-AGATCTAGATCT (RY12) with alternate oligopurines and oligopyrimidines tracts and its homologous 28 bp hairpin oligomer (RY28) that forms a triple helix only in the presence of a pentapeptide REWER. An intermolecular triplex is formed by the single strand invasion of the RY28 duplex by RY12 in the presence of REWER. 5'- oligopurine end of RY12 binds to oligopurine s...
tinely isolate 80-100 yg of RNA per 8 mL of culture. RNA isolated by this method or with TRIzol (manufacturer's recommended procedure) exhibited an A260-280 ratio of 1.6-1.8. When needed, RNA samples were supplemented with RNA loading dye [0.72 mL formamide, 0.16 mL lox MOPS buffer, 0.26 mL 37% formaldehyde, 0.18 mL distilled water (DEPC-treated), 0.10 mL 80% glycerol, and 8.0 mg bromophenol bl...
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