Objective Bacterial penetration of dentinal tubules via exposed dentine can lead to root caries and promote infections of the pulp and root canal system. The aim of this work was to develop a new experimental model for studying bacterial invasion of dentinal tubules within the human oral cavity. Material and Methods Sections of human root dentine were mounted into lower oral appliances that wer...

OBJECTIVE A morphological and ultra-structural study of copper vapor laser (lambda = 510.6 nm) effects on enamel and dentine was performed to show the effects of this radiation. METHODS A total of 15 human molars were cut in half; 15 pieces were separated for irradiation on enamel and 15 for dentine. These two groups were further divided into five experimental groups, including a control grou...

This study was performed to examine the possibility of Er:YAG laser for dentine hypersensitivity treatment using a novel laser probe; broom-type probe. The morphological change of dentinal tubules of bovine dentine plate after low power laser irradiation (5 or 10 mJ/ pulse, 10 pps) or boiling was observed by SEM. Fifty teeth from 13 patients aged 31–54 years with complaint of dentine hypersensi...

Data have been presented indicating that changes occur in the dental structures of growing albino rats fed diets adequate except for extreme restriction of inorganic salts (Arnim et al.2). Marked rarefaction of the alveolar bone and the formation of irregular dentine containing few tubules, many spaces, and occasional cells were observed. This unusual dentine was most prominent in the cusps of ...

Root caries prevention has been a challenge for clinicians due to its special anatomical location, which favors the accumulation of dental plaque. Researchers are looking for anti-biofouling material to inhibit bacterial growth on exposed root surfaces. This study aimed to develop polydopamine-induced-polyethylene glycol (PEG) and to study its anti-biofouling effect against a multi-species cari...

A method for the preparation of circumpulpal dentine for lead analysis is described. The technique consists of cutting 6oo-,L-thin sections of deciduous teeth, and, utilizing a vice, fracturing off the circumpulpal dentine from the coronal dentine. Both dentine and circumpulpal dentine of teeth of normal American children and previously identified lead-poisoned children were prepared in this fa...

AIM The aim of the study was to assess the efficacy of chemo-mechanical method for caries removal based on histological analysis and scanning electron microscopy of dentine samples. METHODS Forty-five freshly extracted human deciduous and permanent teeth with dentinal caries were used in this in vitro study. The teeth were allocated to two different technique groups. Chemo- mechanical caries ...

Bioactive growth factors identified within the extracellular matrix of dentine have been proposed roles in regulating the naturally inherent regenerative dentine formation seen in teeth in response to trauma and infection, which may also be harnessed for novel clinical treatments in augmenting mineralised tissue repair. This study examined the specific biological action of demineralised dentine...

OBJECTIVE To evaluate the flow dynamics of dentine fluid using a chemiluminescence method in vitro. MATERIALS AND METHODS Horizontally sliced coronal dentine specimens with thicknesses of 1.4, 1.6, 1.8, and 2.0mm (n=10 each) were prepared from extracted human third molars. After cleaning with EDTA, a mounted specimen was clamped between 2 acrylic chambers attached to both the occlusal and pul...

The effects of sealing infected carious dentine below dental restorations on the phenotypic and genotypic diversity of the surviving microbiota was investigated. It was hypothesized that the microbiota would be subject to nutrient limitation or nutrient simplification, as it would no longer have access to dietary components or salivary secretion for growth. The available nutrients would be limi...