OBJECTIVES Mesenchymal stem cells or "multipotent stromal cells" are heterogeneous cell population with self-renewal and multilinage differentiation. The aim of this study was to examine and compare the expression of important stem cell surface markers on two populations of mesenchymal stem cells, one derived from human exfoliated deciduous teeth and the other derived from human adipose tissue....

Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering...

Recent studies have revealed the existence of stem cells in various human tissues including dental structures. We aimed to establish primary cell cultures from human dental pulp and periodontal ligament, to identify multipotential adult stem cells in these cultures, and to study the differentiation capacity of these cells to osteogenic and to neuronal fates. Dental pulp and the periodontal liga...

89 The first document of the American Academy of Pediatric Dentistry, “Policy on Stem Cells”, published in 2008, was aimed at providing dentists with information about the possible use of dental stem cells of their patients Stem cells constantly differentiate and create new specialised cells to replace the dead ones and to repair and regenerate tissues. Adult stem cells can be found in every ti...

Dentin-pulp complex regeneration is a promising alternative treatment for the irreversible pulpitis caused by tooth trauma or dental caries. This process mainly relies on the recruitment of endogenous or the transplanted dental pulp stem cells (DPSCs) to guide dentin-pulp tissue formation. Platelet-derived growth factor (PDGF), a well-known potent mitogenic, angiogenic, and chemoattractive agen...

Recently, numerous types of human dental derived‐mesenchymal stem cells (MSCs) have been isolated and characterized, including dental pulp stem cells, stem cells from exfoliated deciduous teeth, periodontal liga‐ ment stem cells, dental follicle progenitor cells, alveolar bone‐derived MSCs, stem cells from apical papilla, tooth germ progenitor cells, and gin‐ gi...

Background Recently, the clinical use of low power lasers has increased, and it is said that wound healing is accelerated by their irradiation. The aim of this study was evaluation of the effect of platelet-rich plasma on proliferation and differentiation of human dental pulp stem cells with or without Ga-Al-As laser. Methods: In this experimental study, human lower third molar dental pulp c...

Objectives: Success of regenerative dental procedures highly depends on their acceptance by the clinicians; yet, little is known about dental residents’ attitudes regarding this new technology. The purpose of this study was to assess the knowledge and attitude of post-graduate dental students towards stem cells and regenerative dentistry as part of future dental treatments. Methods: This cross...

AIM To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. METHODOLOGY Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evalu...