This study investigated the anticonvulsant activity and possible mechanism of action of an aqueous solution of Dorema ammoniacum gum (DAG) which has been used traditionally in the treatment of convulsions. In this study, the anticonvulsant activity of DAG was examined using the pentylentetrazole (PTZ) model in mice. Thirty male albino mice were divided randomly and equally to 5 groups, and pret...

In the last decade, scheduling of Directed Acyclic Graph (DAG) application in the context of Grid environment has attracted attention of many researchers. However, deployment of Grid environment requires skills, efforts, budget, and time. Although various simulation toolkits or frameworks are available for simulating Grid environment, either they support different possible studies in Grid compu...

When ram spermatozoa were treated with Ca2+ and the ionophore A23187 to induce acrosomal exocytosis, a rise in diacylglycerol (DAG) mass was observed, concomitant with a rapid breakdown of [32P]P1-labelled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate and a rise in [32P]Pi-labelled phosphatidate. Inclusion of the DAG lipase inhibitor RHC 80267 resulted in further bu...

The mechanism of receptor-induced activation of the ubiquitously expressed family of mammalian canonical transient receptor potential (TRPC) channels has been the focus of intense study. Primarily responding to phospholipase C (PLC)-coupled receptors, the channels are reported to receive modulatory input from diacylglycerol, endoplasmic reticulum inositol 1,4,5-trisphosphate receptors and Ca2+ ...

INTRODUCTION The potentially differential roles of both forms of ghrelin in obesity are undefined, and little is known about desacyl ghrelin's (DAG) regulation by meals. We aimed to assess changes in acyl ghrelin (AG) and DAG in response to mixed-meal consumption in obese and non-obese subjects. MATERIAL AND METHODS Venous blood for plasma glucose, AG and DAG assays were collected in both gro...

We recently reported that DAG (diacylglycerol) generated during sphingomyelin synthesis plays an important role in protein kinase C activation and cell proliferation in Madin-Darby canine kidney cells [Cerbon and Lopez-Sanchez (2003) Biochem. J. 373, 917-924]. In yeast cells, IPC (inositol phosphoceramide) synthase catalyses the transfer of phosphoinositol from phosphatidylinositol to ceramide ...

belief-network inference Adnan Darwiche and Gregory Provan Rockwell Science Center 1049 Camino Dos Rios Thousand Oaks, CA 91360 fdarwiche, [email protected] Abstract We describe a new paradigm for implementing inference in belief networks, which consists of two steps: (1) compiling a belief network into an arithmetic expression called a Query DAG (Q-DAG); and (2) answering queries using...

A hybrid blockchain structure (hybrid directed acyclic graph, or H-DAG) is proposed in this article to solve the existing problem of architectures using symmetric key encryption technology by combining characteristics single-chain blockchains and DAG distributed ledgers. By improving block transaction structures optimizing consensus mechanism, H-DAG confirmed orders while maintaining high-throu...

The Escherichia coli sn1,2-diacylglycerol (DAG) kinase has been successfully expressed in COS cells. The E. coli dgkA locus which contains the coding sequences for DAG kinase was subcloned into an eukaryotic expression vector, pMT2. COS cells transfected with the vector pMT2dgk expressed the DAG kinase as shown by Western analysis. Immunofluorescence studies revealed that the E. coli DAG kinase...

Changes in the cellular content of 1,2-diacylglycerol (DAG) in isolated rat pancreatic acini in response to agonist stimulation were studied using a sensitive mass assay. When acini were stimulated by 10 nM COOH-terminal cholecystokinin-octapeptide (CCK8), the increase in DAG was biphasic, consisting of an early peak at 5 s and a second, larger, gradual increase that was maximal by 15 min. The ...