Abstract Background Cytoplasmic male sterility (CMS) is a trait associated with non-functional pollen or anthers, caused by the interaction between mitochondrial and nuclear genes. Findings A Tadukan-type CMS line (TAA) restorer (TAR) were obtained successive backcrossing Oryza sativa cultivars Tadukan (a cytoplasmic donor) Taichung 65 recurrent parent). Using Illumina HiSeq, we determined whol...