The performance of thermal cyclers for polymerase chain reactions (PCR) is of great concern in terms of the reliability of PCR-based assays, particularly when rapid cycling conditions are applied to small volume reactions. In this work, the precision of the temperature controls during rapid thermal cycling was measured in 19 commercial thermal cyclers of 8 different models. The temperatures of ...

The stability of resonant frequency for single wafer, thin film encapsulated silicon MEMS resonators was investigated for both long term operation and temperature cycling. The resonant frequencies of encapsulated resonators were periocidcally measured at 25°C ± 0.1°C for > 9,000 hours, and the resonant frequency variation remained within the measurement uncertainty of 3.1 ppm and 3.8 ppm. Also,...

Uncooled microcantilever-based infrared (IR) detectors have recently gained interest due to their low noise equivalent temperature difference (NETD), while concurrently maintaining low costs. These properties have made them available for a wider range of applications. However, the curvature induced by residual strain mismatch severely compromises the device’s performance. Therefore, to meet per...

s of papers 91 yolk (YP), Haugh units (HU), yolk index (YI), shell percent (SP), shell thickness (ST) and egg specific gravity (ESG). The vitamin D influenced the parameters HU, YI, SP, ST, ESG when in thermoneutral temperature, the YI in a cycling heat stress and ST, ESG in constant heat stress. The vitamin C interfered on HU, SP, ST, ESG under thermoneutral temperature and on YI under cycling...

By means of x-ray diffraction we show that several sodium salts and the disaccharides sucrose and trehalose strongly accelerate the formation of cubic phases in phosphatidylethanolamine (PE) dispersions upon temperature cycling through the lamellar liquid crystalline-inverted hexagonal (Lalpha-HII) phase transition. Ethylene glycol does not have such an effect. The degree of acceleration increa...

We report experimental data of creep cavity shrinkage for dispersion-strengthened-cast aluminum with about 23 vol pct submicron Al2O3 dispersoids, annealed isothermally or subjected to thermal cycling without applied stress. Thermal cycling is found to increase the rate of densification by a factor of 3 to 5.5 relative to isothermal annealing at the upper cycling temperature, allowing for recov...

Failure mechanisms exposed by environmental accelerating testing methods such as thermal cycling or thermal shock test, may differ from those at the service operating conditions. While the device is heated up or cooled down evenly on its external surface during environmental testing, real operating powered devices experience temperature gradients caused by internal local heating, components’ di...

The involvement of chromatin remodelling in dormancy cycling in the soil seed bank (SSB) is poorly understood. Natural variation between the winter and summer annual Arabidopsis ecotypes Cvi and Bur was exploited to investigate the expression of genes involved in chromatin remodelling via histone 2B (H2B) ubiquitination/de-ubiquitination and histone acetylation/deacetylation, the repressive his...