The proliferation of embryonic cells or adult stem cells in tissue is critically regulated during development and repair. How limited the proliferation of cells, so far, is not much explored. Cell-cell contact proliferation inhibition is known as a crucial mechanism regulating cell proliferation in vitro and in vivo. In this study we examined the characters of mouse subcutaneous adipose derived...

the effect of geometry and material of the micro/nano particle on contact mechanic for manipulation was studied in this work based on atomic force microscopy. hertz contact model simulation for eph biological micro particle with spherical, cylindrical, and circular crowned roller shape was used to investigate the effect of geometry on contact simulation process in manipulation. then, to validat...

acanthamoeba keratitis is a vision-threatening infection caused by pathogenic species of the genus acanthamoeba. in this study, 13 acanthamoeba keratitis cases were diagnosed among 52 keratitis patients. to confirm the identity of acanthamoeba at the genus level, a pcr-based method was used, and their pathogenic potential was determined using in vitro cytotoxicity assays on human corneal epithe...

tissue engineering is defined as the designing and engineering of structures to rebuild and repair a body damaged tissue. scaffolding poly hydroxy butyrate valraty (phbv) has shown good biocompatibility and biodegradable properties. nanofibers have improved the performance of biomaterials, and could be considered effective. one of the important methods for designing nanofiber scaffold is the el...

Background and Aim: The purpose of this study was to investigate the formation of gap junctions and to characterize the expression of Connexin (CX) 43 during the proliferation of Malassez’s epithelial rest (MER) cells. Materials and Methods: MER cells derived from the porcine periodontal ligament were cultured in 35 mm dishes as 2D culture. MER cells mixed with type 1 collagen gels were also cu...

Wallace CS, Truskey GA. Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-mediated endothelial cell activation. Am J Physiol Heart Circ Physiol 299: H338–H346, 2010. First published May 21, 2010; doi:10.1152/ajpheart.01029.2009.—We used a direct-contact endothelial cell-smooth muscle cell (EC-SMC) co-culture to examine whether quiescent SMCs regulate the EC infl...

Bone regeneration required suitable scaffolding materials to support the proliferation and osteogenic differentiation of bone-related cells. In this study, a kind of hybridized nanofibrous scaffold material (CNF/BG) was prepared by incorporating bioactive glass (BG) nanoparticles into carbon nanofibers (CNF) via the combination of BG sol-gel and polyacrylonitrile (PAN) electrospinning, followed...

Cell surface lectin receptors underwent rapid redistribution after embryonic Xenopus myotomal muscle cells were manipulated into contact in culture. Soybean agglutinin (SBA) receptors became highly concentrated at the contact area and concanavalin A (Con A) and ricin receptors were depleted at the same region. The accumulation of SBA receptors was greatly reduced by the presence of SBA specific...

Background: Bone marrow-derived mesenchymal stem cells (MSCs) have emerged as beneficial cellular vehicles for nervous system rescue and repair. A better understanding how MSCs are involved in mediating neural repair will facilitate development of novel therapeutic strategies. Methods: In the present study bone marrow-derived MSCs were isolated and characterized from Brown Norway rats. The capa...

BACKGROUND/AIMS Peritoneal fibrosis is a frequent complication of peritoneal dialysis that follows inflammation. It is recognized that epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs), plays a key role in fibrogenesis. However, the relationship between inflammatory macrophages and PMCs remains elusive. In this study, we investigated the effects of different polariz...