linum album l. is containing pharmaceutical composition podophyllotoxin(ptox) which is currently being used as a precursor to semi-synthetic anticancer drugs etoposide, teniposide and etopophos. cell culture is as an important source for the production of ptox. several strategies, including treatment with elicitors have shown for enhanced production of secondary metabolites in cell culture syst...

Background Specific growth factors and feeder layers seem to have important roles in in vitro embryonic stem cells (ESCs) differentiation. In this study,the effects of bone morphogenetic protein 4 (BMP4) and mouse embryonic fibroblasts (MEFs) co-culture system on germ cell differentiation from mouse ESCs were studied. MaterialsAndMethods Cell suspension was prepared from one-day-old embryoid bo...

Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cu...

Summer time is vacation time—a chance to step away from minipreps or grant deadlines and investigate nature outside the lab. For many of us, that means a trip to the beach. But don't let those colorful umbrellas and vigilant lifeguards fool you. Every foray into nature is ultimately a battle between predator and prey. In this Cell Culture, we explore cellular and molecular mechanisms used by pr...

On June 11 th , the best soccer players from around the world will meet in South Africa at the FIFA 2010 World Cup. To reach this level of competition, these players combine phenomenal ball-handling skills with three major characteristics: fast sprinting, rapid reaction times, and ferocious tenacity. This issue's Cell Culture explores these three athletic qualities from a molecular and cellular...

Background: Several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (MSCs) in allogeneic or mitogenic interactions. Cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. Objective: To investigate if adipose derived MSCs could inhibit Jurkat lym-phoblastic leukemia T cell proliferation during coculture. Me...

cell lines derived from mammalian are dominant systems for the production of recombinant therapeutic proteins because of their capacity for correct protein folding, assembly and post-translational modification. in the search of an efficient method for the production of  a recombinant protein using animal cell culture, we investigated the effects of different treatment including fetal calf serum...